Krijger JJ, Horbach R, Behr M, Schweizer PB, Deising HGR, Wirsel S. The yeast signal sequence trap identifies secreted proteins of the hemibiotrophic corn pathogen Colletotrichum graminicola. Mol Plant-Microbe
Signal2sequence trap in mammalian and yeast cells : a comparison. J Membr Biol , 2001 , 183 (3) : 175～182G. Galliciotti, H. Schneider, L. Wyder, A. Vitaliti, M. Wittmer, M. Ajmo, R. Klemenz, Signal-sequence trap in mammalian and yeast cells: a comparison, J. Membr. Biol. ...
Sign up with one click: Facebook Twitter Google Share on Facebook YSST (redirected fromYeast-Based Signal Sequence Trap) AcronymDefinition YSSTYet Somehow Still Typing YSSTYMCA Seahorse Swim Team YSSTYeast-Based Signal Sequence Trap(molecular biology) ...
The trigger signal response of the sensor cells is coupled to the secretion of α-factor, which leads to EGFP expression in the actor cells. A model has been established that simulates the cooperative signal output of a two-cell system. This model aims to achieve a digital response that ...
Page 23 of 41 Matchmaker Gold Yeast Two-Hybrid System User Manual C. Protocol: Distinguishing Genuine Positive from False Positive Interactions Y2HGold Yeast Strain contains four reporters under the control of three distinct GAL4 UAS sequences. As a result, of following ...
Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed,...
yeast two-hybrid system Yeast Ubiquitin Hydrolase Yeast Ubiquitin Hydrolase 1 Yeast-Based Signal Sequence Trap Yeast-bitten Yeast-Extract Dextrose Yeast-Extract Mannitol Yeast-Extract Peptone Adenine Dextrose yeast-like yeast-like yeast-like Yeast-Nitrogen-Glucose yeasted yeasted yeasted yeaster yeastier...
We discuss the advantages of using Saccharomyces cerevisiae for signal sequence trap technology. The yeast protein-translocation system appears to be less discriminating than that of higher eukaryotes – for example, a Theileria parva cysteine protease gene containing a recessed, nonclassical signal allows...
Both systems report disrupted interaction by a gain of signal which is easier to detect in a library screen as compared to a loss of signal. The Split-ubiquitin system (Figure 2D) was designed by Johnsson and Varshavsky in 1994 [53] to allow detection of protein-protein interactions occurring...
trap barriers Single cell analysis of yeast replicative aging Mechanical cell patterning in elongated cavities Monitoring the dynamics of cell division Mechanical cell patterning using trap barriers Studying ageing and dynamic single-cell responses Patterning in microcavity array by negative pressure, and ...