Krijger JJ, Horbach R, Behr M, Schweizer PB, Deising HGR, Wirsel S. The yeast signal sequence trap identifies secreted proteins of the hemibiotrophic corn pathogen Colletotrichum graminicola. Mol Plant-Microbe
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et al. Signal-sequence trap in mammalian and yeast cells: a comparison. J Membr Biol 183, 175–182 (2001). 23. Lee, S. J. & Rose, J. K. A yeast secretion trap assay for identification of secreted proteins from eukaryotic phytopathogens and their plant hosts. Methods Mol Biol 835, ...
Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed,...
Thus, the new MYTH 2.0 bait vector pTLB-1 comprises a very strong TEF1 promoter, a LEU2 gene, a Kozak initiation signal sequence, an inverted cassette of pTMBV1 with the composites (LexA+VP16)-Cub-Linker-MCS, and additional stop codons guaranteeing termination (see Fig. 1B). The ...
Recognition signal for C-mannosylation of Trp-7 in RNase 2 consists of sequence Trp-x-x-Trp. Mol. Biol. Cell 9, 301–309 (1998). Article CAS PubMed PubMed Central Google Scholar Albuquerque-Wendt, A., Hütte, H. J., Buettner, F. F. R., Routier, F. H. & Bakker, H. ...
cell cycle progressionis a coordinating signal that is essential for ubiquitination and degradation of major cell cycle regulating proteins such as cyclins and cyclin-dependent kinase inhibitors (eg, yeastSic1, human p27Kip1)19Protein misfoldingis another common initiating signal for ubiquitination, ...
A signal-anchor sequence stimulates signal recognition particle binding to ribosomes from inside the exit tunnel. Proc. Natl Acad. Sci. USA 106, 1398–1403 (2009). Article ADS CAS PubMed PubMed Central Google Scholar Mariappan, M. et al. A ribosome-associating factor chaperones tail-anchored...
trap barriers Single cell gene expression analysis Robotic cell printing Localisation of the yeast proteome during polarised growth Mechanical cell patterning in microfluidic microchambers Quantitative analysis of the yeast pheromone signalling response Patterning on adhesive micropatterns Microcontact printing of ...
Technique Split-GFP system [111–115,236] Synthetic genetic array [237] Yeast two-hybrid [238] Used for Protein–protein interaction Synthetic lethality Protein–protein interaction Prion-forming assay [233] Prion forming Yeast transcriptional reporting aggregating proteins (yTRAP) [235] Prion forming...