Fluorescence microscopy of sfGFP expression was performed using an Olympus FV1200 optical microscope (IX81) with a 60× objective lens. Flow cytometry Cells were incubated and treated as described above. The yFYV9 (PADH1-sfGFP) cells were cultured for different time points (2, 3, and 4 h)...
Li et al.32 extensively reviewed methods for analyzing microscope images of microorganisms, where he focused on techniques for classifying microorganisms using different image processing steps like preparation, feature extraction, classification, and evaluation. Rea et al.33develops a GPU-based software us...
Fluorescence microscopy images were acquired as multiplane z-stack images at 0.2 μm intervals over 6 μm on an inverted fluorescence microscope (Olympus IX81) equipped with an electron-multiplying CCD camera (ImagEM C9100-13) and 150× objective lens (UAPON 150× OTIRF, NA/1.45; Olympus)....
Here we demonstrate a new method to monitor the yeast cell concentration using our previous developed microscope-portable microscopy based on fiber-optic array (FOA). The sample is illuminated by a broadband LED and a light diffuser is used to create uniform illumination over the...
Label-free Raman imaging has proven powerful for studying such dynamic behaviors in vivo and at the molecular level. To construct Raman images, univariate data analysis has been commonly employed, but it cannot be free from uncertainties due to severely overlapped spectral information. Here, we ...
The number of living cells (method 1) was determined under the microscope BX51TF1 (Olympus Optical Co. Ltd) using the Thoma neu chamber (Paul Manrenfeld GmbH & Co. KG Lauda-Konigshofen). To facilitate cell counting, the yeast suspension samples were prepared with an aqueous solution ...
The samples were examined with a fluorescence microscope (DM 6000 B, Leica Microsystems, Germany) using a green fluorescence filter (546 nm). Images were taken under 40 × objective for randomly selected transformant and processed with imaging software OCULAR. 2.8. Quantitative real-time PCR ...
Relatively good binary image was obtained by using white LED as microscope light source, but uneven brightness distribution in original image hindered counting accuracy. A block binarization method using local thresholds proportional to local brightnesses was used to get improved binary images. The ...
The mechanism of action of antimicrobial peptides (AMP) was initially correlated with peptide membrane permeation properties. However, recent evidences indicate that action of a number of AMP is more complex and involves specific interactions at cell env
In diploid species, genetic loci can show additive, dominance, and epistatic effects. To characterize the contributions of these different types of genetic effects to heritable traits, we use a double barcoding system to generate and phenotype a panel of