Notably, the integration of the innovative You Only Look Once (YOLOv5) facilitates automatic cell detection within a finely partitioned grid system. This leads to the development of two models—one operating on pristine raw images, the other harnessing the enriched landscape of FACE-enhanced ...
In the calcofluor white stain, a fluorescent compound binds to certain cell wall polysaccharides found in all fungi; organisms stained by this dye can be readily visualized with the use of a fluorescence microscope. Among the procedures that can be used for visualization of P. jirovecii are ...
Cell adhesion on sensors can be influenced by the use of different sensor materials or material coatings [1]. The cell-materialinteraction can be characterized, e.g., by the adhesion force. Additionally, filter blocking on sampling devices for large scale bioreactors can be avoided under ...
Under a Creative Commons license Open accessExtracellular vesicles (EVs) play important roles in cell-cell communication. In budding yeast (Saccharomyces cerevisiae), EVs function as carriers to transport cargo proteins into the periplasm for storage during glucose starvation. However, intracellular organe...
yeast cells can be made visible in the living cell with an ordinary light microscope.Many of the structures in yeast cells can be stained in temporary liquid mounts of unfixed cells by control of pH, oxidation-reduction potential and the ratio of cell concentration to the concentration of dye....
the cell suspension was applied onto a glass slide and dried at room temperature. 10 μl of DAPI-containing Vectashield Mounting Medium (Vector Laboratories, #H-1200) was added before sealing with a coverslip. Chromosomes were observed under a DeltaVision deconvolution microscope (GE Healthcare)...
After 20 min staining, the numbers of viable (living) cells and of total cells were counted under a microscope (Nikon Ci-L). The cell viability and mortality are calculated according to the following equation: Detox fermentation of glucose Fermentation broths were prepared in 100 or 250 ...
Robust Cellular StructureYeast cells have a robust cellular structure that allows them to withstand environmental stress and thrive under industrial fermentation conditions. Their resilience makes them well suited for large-scale production processes, including high-stress environments such as high cell densi...
Yeast propagation occurs under aerobic conditions, and cells convert oxygen and sugars into carbon dioxide and energy through oxidative metabolism to allow efficient yeast cell growth. Under anaerobic conditions, such as those used in beverage and fuel ethanol production, yeasts are much less efficient...
(Kaeberlein, 2010). RLS is classically assayed by micro-dissecting yeast cells on a nutrient-rich agar plate under a microscope; typically, more than 20 “virgin” or undivided cells are separated for each strain to be tested, then these cells are allowed to divide. After division, daughter...