In the era of artificial intelligence and machine learning, these cutting-edge approaches are now widely applied in biological image processing, such as cell type identification, cell count, cell structure analysis, and cell reconstruction3. On the other hand, the effectiveness of image classification...
Published evidence suggests that, under some conditions, yeast can recover from the loss of membrane integrity that allows PI to enter the cell [1,2]. In such a case, yeast viability calculations that rely on...
Published evidence suggests that, under some conditions, yeast can recover from the loss of membrane integrity that allows PI to enter the cell [1,2]. In such a case, yeast viability calculations that rely on ...
in general, yeast is man's friend. Yeast is used to ferment the sugars of various grains to produce alcoholic beverages and in the baking industry to expand dough. But in the world of mammalian cell culture, yeast
In the calcofluor white stain, a fluorescent compound binds to certain cell wall polysaccharides found in all fungi; organisms stained by this dye can be readily visualized with the use of a fluorescence microscope. Among the procedures that can be used for visualization of P. jirovecii are ...
Then, the culture medium was decanted, and cell pellets were resuspended in the volume of medium that remained and immediately observed under a confocal fluorescence microscope. Images were taken using a motorized Olympus IX81 microscope (Olympus Corporation, Tokyo, Japan) equipped with a Yokogawa ...
Bartender: a fast and accurate clustering algorithm to count barcode reads. Bioinformatics 34, 739–747 (2018). Article CAS PubMed Google Scholar Li, F., Salit, M. L. & Levy, S. F. Unbiased fitness estimation of pooled barcode or amplicon sequencing studies. Cell Syst. 7, 521–525....
(Fig.5D, compare day 0 with day 4 or day 12). We noticed that cell count decreased over the same period of time, by an average of 2- and 6.7-fold at day 4 and day 12 (see Additional file3), suggesting that cell lysis was occurring and that release of intracellular MHETase could...
Published evidence suggests that, under some conditions, yeast can recover from the loss of membrane integrity that allows PI to enter the cell [1,2]. In such a case, yeast viability calculations that rely on...
Published evidence suggests that, under some conditions, yeast can recover from the loss of membrane integrity that allows PI to enter the cell [1,2]. In such a case, yeast viability calculations that rely on...