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After 20 min staining, the numbers of viable (living) cells and of total cells were counted under a microscope (Nikon Ci-L). The cell viability and mortality are calculated according to the following equation: Detox fermentation of glucose Fermentation broths were prepared in 100 or 250 ...
In extended-fiber fluorescence in-situ hybridization (FISH), the location of a specific gene on a chromosome is determined by stretching the chromosome to a straight fiber and observing the binding of a fluorescence-labeled oligonucleotide under a microscope. In this paper, we propose and ...
Digestion of asci was followed by examining aliquots under a microscope, and the reaction was stopped by placing the samples on ice and adding 150 μl of sterile H2O. A small amount of cells was transferred to a strip of YPD agar on a sterile microscope slide for tetrad analysis. Spores ...
This study is specifically centred on assessing performance under the condition of K = 3. However, it is imperative to acknowledge the need for future investigations to ascertain the optimal clustering parameter. Furthermore, it becomes evident that achieving a brighter photograph with enhanced ...
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to ...
Transient and steady-state populations of each organism in mixed culture at various dilution rates were enumerated with hemocytometer under a microscope. The difference in size of the organisms permitted easy resolution. The essential nutrilite, nitrogen, which is fixed by the bacteria and required by...
Most of the structures which have been described in yeast cells can be made visible in the living cell with an ordinary light microscope.Many of the structures in yeast cells can be stained in temporary liquid mounts of unfixed cells by control of pH, oxidation-reduction potential and the rati...
Contamination checks for bacteria or other non-yeast microbes were performed regularly by observing the cultures under a microscope. Library preparation Frozen cultures were thawed and DNA was extracted for library preparation using the MasterPure Yeast DNA Purification kit. Any remaining RNA was removed...
We designed a PDDI2-down fragment (Fig. 4a) which partially drove the expression of the essential gene under the induction of cyanamide. Thus, only adding cyanamide can ensure the cell viability during the promoter replacing process, and an extra wild-type copy of the target gene is ...