Most yeasts are generally recognized as safe (GRAS), easy to be genetically modified, and easy to separate in downstream processing because of their relatively large size. S. cerevisiae is the most studied of simple eukaryotes. It is the first eukaryote with its genome completely sequenced and ...
Genome, FungalOpen Reading FramesPromoter Regions, Geneticp pBackground/p pThe regulatory information encoded in the DNA of promoter regions usually enforces a minimal, non-zero distance between the coding regions of neighboring genes. However, the size of this minimal regulatory space is not ...
Minimal inter-CDS length is correlated with genome size and total intergenic space In the presence of either a bias towards deletions or selection for genome compactness, we expect a strong correlation between total intergenic space and minimal promoter size. Furthermore, as genome size is the sum...
While all of the 16 yeast nuclear chromosomes are well reconstructed by every tested pipeline with respect to continuity, accuracy and completeness, many pipelines failed to reconstruct the mitochondrial genome (Supplementary TablesS5andS6). This chromosome is the smallest one in the reference assembly,...
Full size image Taken together, these results indicate that the genome assemblies generated by the combined assembly approach are structurally correct, accurate, and complete, although telomeres, repeat elements, and ploidy remain a challenge to accurately reproduce. This is currently a challenge in the...
GD offers significant improvement over the manual inspection method to detect relative yeast colony size differences. The speed and accuracy associated with GD makes it an ideal choice for large-scale functional genomics investigations.Background The increasing number of completed genome sequencing projects...
We have carried out a haploinsufficiency (HI) screen in fission yeast using heterozygous deletion diploid mutants of a genome-wide set of cell cycle genes to identify genes encoding products whose level determines the rate of progression through the cell cycle. Cell size at division was used as ...
microbial life using genome data, and, hence, contribute also to our knowledge of metabolic potential (Bodor et al.2020; Lücking et al.2021). Here, we present two cases of metabarcoding studies that highlight how this approach contributed to our understanding of the diversity of soil-borne ...
oCluster analysis and display of genome-wide expression patterns (1998)by M. B. Eisen, P. T. Spellman, P. O. Brown and D. Botstein: This paper describes clustering of genes. The results of this paper showed that the 5 different classes Brown et Al. are trying to predict more or les...
Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints Feiran Li, Yu Chen, Qi Qi, Yanyan Wang, Le Yuan, Mingtao Huang, Ibrahim E. Elsemman, Amir Feizi, Eduard J. Kerkhoven & Jens Nielsen Nature Communications volume 13, Article...