Genome-sequencing program provides enormous data on genes, proteins, and metabolic pathways within the fungal kingdom, thereby helping in solving the long awaited problems of the medical science, ecological science, bioenergy, and several fermentation and enzyme-based biotech industries. Interestingly, ...
Design of a synthetic yeast genome We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromoso... SM Richardson,LA ...
1 | Access to the yeast genome is limited in nuclei. a, Genomic DNA is only partially methylated by Dam in nuclei. Nuclei were treated with increasing concentrations of Dam (0, 0.77, 1.5, 2.9, 5.6nM); purified genomic DNA was digested with DpnI. Electrophoretic analysis in a 1% agarose ...
genome. We show this by assembling diverse engineered yeasts, in some cases revealing unintended deletions and integrations. Furthermore, the resulting whole genomes are high quality, although the underlying assembly software does not consistently resolve highly repetitive genome features. Finally, we ...
Present address: Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany Martin Štefl Present address: Department of Biophysical Chemistry, J. Heyrovsky Institute of Physical Chemistry of the CAS, Prague, Czech Republic Anton Khmelinskii Present address: Institute of ...
The genome sequence of the yeast Saccharomyces cerevisiae has provided the first complete inventory of the working parts of a eukaryotic cell. The challenge is now to discover what each of the gene products does and how they interact in a living yeast cell. Systematic and comprehensive approaches...
The ambitious plan, which they have dubbed the Synthetic Yeast Genome Project or Sc2.0, is a collaboration with fellow Johns Hopkins University researchers Joel Bader and Srinivasan Chandrasegaran. The group recently reported the replacement of one 90-kilobase arm of chromosome IX (synIXR) and a...
Chromatin-associated genes protect the yeast genome from Ty1 insertional mutagenesis. Chromatin-associated genes protect the yeast genome from Ty1 insertional mutagenesis. Genetics. 2008; 178 :197–214. doi: 10.1534/genetics.107.082602. [ ...
We further find that young, species-specific regions are on average much longer than older regions, suggesting either a bias towards deletions or selection for genome compactness in yeasts. While the length evolution of promoter-less intergenic regions is well described by a simplistic, purely ...
yeastgenome/yeastminemaster 3 branches 0 tags Code Latest commitkkarra change to use 8082 10900f2 Nov 16, 2023 Git stats 51 commits FilesType Name Latest commit message Commit time dbmodel domain enrichment May 4, 2022 17:51 gradle/wrapper save May 12, 2020 10:54 ...