Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous expression of genes of interest in the whole body can be visualized by X-gal staining. This protocol is suitable for examining ...
This detection method is highly sensitive and has the advantage that the results can be observed without the need for specialized microscopy equipment. This protocol describes general procedures for X-gal labeling of neural tissue from Drosophila. 展开 ...
Gene Ther. 8, 1545 (1997); of fixative conditions in X-gal staining: W. Ma et al., J. Histochem. Cytochem. 50, 1421 (2002); of blue-white screening procedure: A. L. Sherwood, BioTechniques 34, 644 (2003). Properties: Amorphous solid from methanol, mp 237-239° (dec). [a]D...
The β-Galactosidase assay withX-galis a straightforward method that can be used to detect gene activity of components of the vitamin A pathway by staining embryos from transgenic mouse lines, such as RARE-LacZ andRdh10βgeo(Sandell et al., 2012, 2007). In the six-step protocol, embryos ...
Whole Mount X-gal StainingProtocol for Whole mount staining of intestinal tissueJared Fischer
X-gal staining, which involves the precipitate formed by the reaction between 尾-gal and X-gal, is usually recognized as a light blue or green reaction product on light microscopic (LM) examination. However, the standard protocol for TEM preparation weakens the intensity of or results in the ...