X-galSkeletonStainingThe lacZ gene, encoding for the 尾-galactosidase enzyme, is widely used as a reporter gene in bone biology due to the ease of visualization in situ on whole-mount or on tissue sections. In this protocol we provide detailed methods for visualizing this reporter gene for ...
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It is used as a mutagen, and also as a marker for DNA synthesis (the incorporation of BUdR can be recognized because the staining pattern differs: an even more sensitive method uses a monoclonal antibody staining procedure.) buffer A system that acts to minimize the change in concentration of...
Applications Reported: Purified Anti-beta galactosidase fusion proteins poly has been reported for use in immunoblotting (WB), and immunohistochemical staining.Applications Tested: Purified Anti-beta galactosidase fusion proteins poly has been tested by immunoblotting (WB). (1:400 starting dilution...
The SA-β-gal staining results of frozen tissue sections showed a significant increase in staining in the D-gal aging model group. Eudesmin exhibited a significant protective effect against pancreatic cell aging (Fig. 4A). Similarly, the immunofluorescence results of the aging-related cell cycle ...
The CT reconstruction protocol, which used a down sampling factor of 1, was set to interpolate bilinearly using a Shepp-Logan filter. Image analysis software (Inveon) was used for visualization and analysis. Bone morphometric analysis The treatment effect of the enzymes was investigated by bone ...
(Merck, Germany) and incubated at 37 °C for 24 h. Initially, the colonies were counted. In cultures with bacterial counts of > 104cfu/ml, the specimens were considered as positive, and gram-staining technique was performed. Then, bacterial genus and species were determined by standard ...
(B) Histochemical staining of SA-βgal activity (blue) in 3-week NOD islet cells cultured in the indicated media. Quantification of SA-βgal+ cells are averages of n = 3 biological replicates per group, error bars are SD. ∗∗p < 0.005, one-way ANOVA with Dunnett’s multiple compa...
To visualize Ltbp2 promoter activity, we performed X-gal staining on ocular tissue from Ltbp2-null mice (Fig. 8). X-gal staining was undetectable at postnatal day 5 (P5; Fig. 8A,B) but was evident at P7 (Fig. 8C) and strong by P21 (Fig. 8D). Expression was restricted to the ...
It may be necessary to select for a particular subpopulation of the originally harvested cells for use in the infection protocol. For example, white blood cells may be separated from red blood cells using an apheresis procedure. The white cells may then be placed in culture with mitogens, ...