Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous expression of genes of interest in the whole body can be visualized by X-gal staining. This protocol is suitable for examining ...
内容提示: X-Gal Staining for Muscle Modified at July 1 2, 1 993 By B.Wang 1 . Fixation: 2 hours at 4 4% paraformaldhyde, 1 % gluteraldehyde 0.01 % NP-40 0.01 % Na deoxylcholate 2. Stain: overnight with x-gal solution at room temperature: 1 00mM Na. Phosphate buffer, pH ...
Detection of 尾-Galactosidase Activity: X-gal Staining Burn SF. Detection of β-galactosidase activity: X-gal staining. Methods Mol Biol Clifton NJ. 2012;886:241-50.Burn, S.F., 2012. Detection of beta... Sally F. Burn 被引量: 25发表: 2012年 [Methods in Molecular Biology] Kidney ...
X-GalStainingforMuscleModifiedatJuly12,1993ByB.Wang1.Fixation:2hoursat4oCwithfixationsolution.4%paraformaldhyde,1%gluteraldehyde0.01%..
This detection method is highly sensitive and has the advantage that the results can be observed without the need for specialized microscopy equipment. This protocol describes general procedures for X-gal labeling of neural tissue from Drosophila. 展开 ...
Gene Ther. 8, 1545 (1997); of fixative conditions in X-gal staining: W. Ma et al., J. Histochem. Cytochem. 50, 1421 (2002); of blue-white screening procedure: A. L. Sherwood, BioTechniques 34, 644 (2003). Properties: Amorphous solid from methanol, mp 237-239° (dec). [a]D...
染色gal移植stainingtransplantationinfluencing ACTAANATOMICASINICAVo.33,No.Jun2002移植研究中X-gal染色的影响因素及优化条件张琪李静雯鞠躬(第四军医大学神经科学研究所,西安710032)[摘要]目的研究在细胞移植过程中,利用X-ga染色定位acZ转基因细胞时,消除宿主内源性-Ga)非特异背景的条件。方法分别取成年及新生大小鼠,...
Alkaline Treatment after X-Gal Staining Reaction for Escherichia coli β-Galactosidase Enhances Sensitivity 来自 NCBI 喜欢 0 阅读量: 32 作者:H Tanahashi,T Tabira 摘要: We approach synchronous collaboration via an objectoriented multi-user programming environment where objects are open, relational, and ...
名称编号规格价格手册 x-Gal Staining Solution for Drosophila eyes6-372610mL995 英文名称:x-gal Staining Solution for Drosophila eyes 运输:4-25℃ 保存:4-25℃ 有效期:1 Year 货期:2-4 days 其他: 产品介绍: Order Online在线订购 订货信息
x-Gal Staining Solution for HSV6-372710mL995 英文名称:x-gal Staining Solution for HSV 运输:4-25℃ 保存:4-25℃ 有效期:1 Year 货期:2-4 days 其他: 产品介绍: Order Online在线订购 订货信息 产品名称* 申请类型请选择类型产品订购试用索取* ...