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The sections were then deparaffinized, prepared for histology, and blocked in staining buffer containing the appropriate IgG control. Immunohistochemistry was performed with citrate or Tris buffer antigen retrieval with the following antibodies: a TG antibody (1:100) and a TSHR antibody (1:200). ...
The standard experimental procedure for all measurements consisted of placing the sensory material in the bottom of a standard cuvette (1 cm side), in MeOH: pH = 7 Buffer. Next, a certain amount of amino acids is added, and the diffusion process of the dye from the sensory material to ...
3.5. Stationary Radiolysis A mixture of BrSdU (10−4 M), 0.03 M t-BuOH (used as a scavenger of the •OH radicals), and phosphate buffer (10 mM, pH = 7.0) was purging with argon for ca. 3 min in order to remove oxygen from the solution. The dose absorbed by all samples ...
建议在高 pH 条件下使用碳酸氢铵缓冲液代替碳酸铵缓冲液,是出于以下考虑: 1) 碳酸铵在水中会转化/分解为氨基甲酸铵和碳酸氢铵。随着碳酸铵浓度的增加,可能会形成中间反应性尿素(更多的氨基甲酸铵)。氨基甲酸铵和尿素会在有水的流动相中积聚。此外,缓冲液中的氨会与水...