This paper describes the production of a universal buffer system which can be used for acidic, neutral and basic mobile phases in HPLC. The mixture of alkylamine and phosphoric acid diluted with water gives the possibility to carry out separations with basic buffers on reversed phase columns with...
Mobile Phase Buffers in Liquid Chromatography: The Buffer–Detector Battle Dwight R. Stoll;Ella Sontowski February 12th 2025 Article In this installment, I primarily discuss considerations to keep in mind when choosing buffering additives that will be used for LC methods invo...
Ammonium hydrogencarbonate was tested for a number of drug-like compounds analysed as mixtures, and data obtained were compared to those from the classical and MS-friendly buffers widely used by chromatographers: trifluoroacetic and formic acids. The results of this study revealed the suitability of...
performed as follows: increasing convertase concentrations (generating from 0.1 to 2.5 nmol of 7-amino-4-methylcoumarin from the fluorogenic substrate pERTKR-MCA) were incubated with 8 pmol of the 1-19 substrate for 1 h at 37°C in 100 μl of buffers identical to those used for gp160 ...
Screeningforbasicdrugsinequineurineusingdirect-injection differential-gradientLC–LCcoupledtohybridtandemMS/MS ShawnM.R.Stanley ∗ ,HsiaoChingFoo TheSingaporeTurfClubLaboratory,SingaporeRaceCourse,1TurfClubAve,738078Singapore,Singapore Received7November2005;accepted10March2006 Availableonline17April2006 ...
A further object of the invention is to provide a ready-to-use kit for the selective detection of tumoral cells. The present invention relates to a basic protein of the phospholipase A2 (PLA2) type, isolated from the venoms of snakes, especially of the family Elapidae, which protein compris...
Following incubation for 20 h at room temperature in the dark, the protein was freeze-dried and then redissolved as specified. Digests were carried out at 37 (trypsin and pepsin) or at 55 °C (thermolysin) in appropriate buffers as specified, using an enzyme/substrate ratio of 1:200–1:...
buffers and all the pH readings were obtained in the pH scale before mixing the aqueous buffer and the organic modifier. For simplicity, the left side notation in pH symbols has been omitted in this paper. The scopolamine hydrobromate trihydrate standard was pur- chased from Acros (NJ, USA...
represented by clonazepam, the organic acid has very limited impact, i.e., the solubility enhancement amounts typically to less than 3-fold. For example, the solubilities of clonazepam are about 11.6 and 6.9 μg/mL in buffers at pH 2.3 and 6.8 containing a higher and lower concentration of...
Examples of carriers, excipients and stabilizers include saline, PBS, buffers such as phosphate, citrate and other organic acids; antioxidants such as ascorbic acid, low molecular weight polypeptides; proteins such as serum albumin, immunoglobulins, gelatins; hydrophilic polymers such as PVP; amino ...