Contact usColumn selection guide Overview The column you need with multiple functionalities Do you want to separate ionic and hydrophobic analytes in a single run? Separate polar, nonpolar, and ionizable compounds in a single analysis and achieve baseline separation of challengi...
The selection of organic solvents, pH, and buffer additives should be carefully considered to improve resolution and minimize peak tailing or broadening. A systematic approach involving trial and error is often employed to find the optimal mobile phase conditions for a specific protein or peptide. ...
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towards acidic conditions. This specialty column exhibits excellent selectivity and high resolution for the aminoglycoside antibiotics analysis. In addition, it is easy to use because simple aqueous mobile phase (e.g. 100 mM TFA) without organic solvent and pH adjusting buffer is sufficient in most...
– LinkedIn Aug 31, 2022 · Two types of applications: The most common vial sizes for liquid chromatography vials are 12 x 32 mm as well as 15 x 45 mm. 12 x 32 mm vials will also be described as 1.5 mL, 1.8 mL, or 2.0 mL Gas Chromatography Syringe Selection Guide syringe selection...
sodium phosphate dibasic anhydrous buffer (65:35 v/v) at pH of buffer 7.0 ± 0.02. It was found that the calculated values for Student’st-test and the variance ratioF-test were less than the tabulated values. The obtained results were summarized in Table2. The results proved that ...
5062-8517 5041-2168 G1322-67300 Mobile Phase Contamination of solvents, especially of aqueous buffer solutions is a common problem in HPLC. Aqueous media are good growth environments for microbes that can clog your HPLC and contribute to background. To test if the solvent inlet filter is ...
• After use with a buffer, purge the pump with HPLC-grade water. • When possible, use eluents of similar viscosity when you run a gradient. • Consider column chemistry. If the column can be damaged by an intermediate or new eluent, remove it from the system, and substitute a ...
We estimated the method greenness using different Green Analytical Chemistry (GAC) metrics such as Analytical Eco-scale, scoring of hazardous values, Analytical Method Volume Intensity (AMVI), Environmental Assessment Tool (EAT), solvent selection guide approach, and Eco-solvent approach....
concurrent reversed-phase, cation-exchange and anion-exchange functionalities for maximum flexibility in method development. Easily optimize selectivity by adjusting mobile phase buffer concentration, pH and solvent content—either jointly or independently—to accelerate chromatographic separations and increase ...