Why is RNA important to the cell? Why does DNA synthesis require an RNA primer? Why is there no helicase in a PCR? Why are nucleotide bases hydrophobic? Why is adenine used for energy more than the other nucleotides? What sugar distinguishes RNA from DNA?
For gene expression assays, the Tm of the probe should be about 10°C above that of the primer to enable the probe to bind to the template strand before Taq polymerase reaches it. Also important are primer annealing temperatures (Tm), which should generally be 58–60°C...
Why is the double helix important for DNA replication? Why is DNA negatively supercoiled? Why are primers needed for DNA replication? Why does DNA replication only occur in the 5' to 3' direction? Why do we do DNA sequencing? How does DNA replication end?
PCR primers can be carefully designed to flank and amplify the hypervariable target region of interest, allowing researchers to be confident that the TCR gene is being accurately targeted for sequencing and capture of the somatic informat...
What are the three steps of PCR? PCR is based on three simple steps required for any DNA synthesis reaction:(1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the...
These are combinations of custom, single-stranded DNA sequences for creating CRISPR libraries, primer pools for multiplex PCR, data storage, or gene construction. Because of the highest coupling efficiency, oPools can be ordered for oligos up to 350 nt in length, the longest pooled oligos ...
Nuclear extracts underwent RT – PCR after immunoprecipitation ('IP') or not ('no IP'), with antibody to CUGBP, nuclear pore proteins, TF Sp1 and PDGF receptor. Anti-CUGBP coprecipitated DMPK RNA only from DM-affected, and not control, cells, using primers (101 – 102) spanning the ...
The resulting single-strand cDNA was used as DNA template for qPCR using primers (Supplementary Table 1) for 26 genes from the RNA-seq data. The EF1A gene was used as the control (Xia, He, Bai, Lin, & Yue, 2011). PCR in triplicates was performed with the KAPA™ SYBR® FAST ...
O€ pik discussed the use of this database and showed that most of the 'virtual taxa' (or OTUs) are known only from environmental sequences. This fact helps to emphasize how poorly this important group of fungi is known. It also underscores the value of a conservative marker, like SSU,...
In these cases molecular approaches are mainly based on PCR assays; however, the performance of these techniques can become compromised, as formalin fixation is associated with DNA damage. Currently, molecular diagnostics for mucormycosis is not widely available; its use is compromised by limited ...