Why are nucleic acids important? Why are primers needed for DNA replication? Why do cells use RNA in protein synthesis, rather than translate directly from DNA? Explain why one strand of DNA can be replicated continuously, while the other has to be made in short segments. ...
In SSP-PCR, why is it important to determine genomic DNA concentration and quality for interpretation of the test results? Is the RNA and DNA polymerase in prokaryotes and eukaryotes the same? If not what are the differences? Explain why RNA primers are used ...
The use of consensus primer and PCR sequencing may even help to identify novel parasitic species. The key limitation of molecular detection is the technological expertise and expense which are usually lacking in the field setting at highly endemic areas. However, such tests can be useful for ...
In these cases molecular approaches are mainly based on PCR assays; however, the performance of these techniques can become compromised, as formalin fixation is associated with DNA damage. Currently, molecular diagnostics for mucormycosis is not widely available; its use is compromised by limited ...
These are combinations of custom, single-stranded DNA sequences for creating CRISPR libraries, primer pools for multiplex PCR, data storage, or gene construction. Because of the highest coupling efficiency, oPools can be ordered for oligos up to 350 nt in length, the longest pooled oligos ...
What are the three steps of PCR? PCR is based on three simple steps required for any DNA synthesis reaction:(1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the...
Nuclear extracts underwent RT – PCR after immunoprecipitation ('IP') or not ('no IP'), with antibody to CUGBP, nuclear pore proteins, TF Sp1 and PDGF receptor. Anti-CUGBP coprecipitated DMPK RNA only from DM-affected, and not control, cells, using primers (101 – 102) spanning the ...
Ethidium bromide-stained 25S rRNA provides the RNA loading control. B. RT-PCR was performed on Col-0, KO and OEX total RNA extracted from leaves of 4 week old plants. Amplified genes are indicated. Semi-quantitative conditions and primers for β-tubulin amplification were used to ensure ...
Why is RNA important to the cell? Why does DNA synthesis require an RNA primer? Why is there no helicase in a PCR? Why are nucleotide bases hydrophobic? Why is adenine used for energy more than the other nucleotides? What sugar distinguishes RNA from DNA?
Why is the double helix important for DNA replication? Why is DNA negatively supercoiled? Why are primers needed for DNA replication? Why does DNA replication only occur in the 5' to 3' direction? Why do we do DNA sequencing? How does DNA replication end?