Quantitative real-time PCR (qPCR) was subsequently performed using SYBR green master mix (Applied Biosystems, Foster City, CA, USA) and gene-specific primers in the Stratagene M × 3000P real-time PCR system (Agilent Technologies). The primers were synthesized by Sangon Biotechnology ...
Inflammatory microenvironment causes the change of epigenetic modification in periodontal ligament stem cells derived from periodontitis tissues (P-PDLSCs), which results in defective osteogenic differentiation compared to cells from healthy tissues. It’s urgent to explore therapeutic strategies aimed at epi...
The sequences of the primers used in this study are shown in Additional file 7: Table S6. H&E staining of tissues Mouse organ tissues were fixed with 4% PFA (Servicebio, Wuhan, China) and dehydrated using a graded ethanol series, followed by clearing with xylene (Shanghai, China). The...
We report a novel loss-of-function mutation in the TLDc domain of the humanOXR1gene, resulting in early-onset epilepsy, developmental delay, cognitive disabilities, and cerebellar atrophy. Patient lymphoblasts show impaired cell survival, proliferation, and hypersensitivity to oxidative stress. These ...
The Gegen Qinlian Decoction (GGQLD) is a renowned traditional Chinese medicinal formula that has been used for centuries to effectively treat asymptomatic Hyperuricemia (HUA). This study aims to investigate the underlying mechanism of GGQLD's therapeutic
The coding sequence of BraA04g009730.3C was amplified in mutant fsm2 plants using the primer sequences shown in Additional file 8: Table S7. Gene cloning was performed according to the method of Huang et al. [92] and samples were sequenced by GENEWIZ (Suzhou, China) using the Sanger meth...
All the primers are shown in Additional file 2. We test the correlation of expression (CEG) between lncRNAs/miRNAs and lncRNAs/mRNAs by using the Pearson correlation coefficient. The Pearson correlation coefficient was calculated by COR() in R [37]. Data access The stranded RNA-seq datasets ...
Trypsin-like serine proteases are involved in a large number of processes including digestive degradation, regulation of developmental processes, yolk degradation and yolk degradome activation. Trypsin like peptidases considered to be involved in digesti
(200 nM) forward and reverse primer/well). All measurements were conducted using the QuantStudio™ 3 Real-Time PCR System (Applied Biosystems) and results were evaluated using the ΔΔCT method as described previously [25]. Actin served as a housekeeping gene. Primers (Table1) were ...
64Citations 56Altmetric Metrics Abstract Dysregulated translation is a common feature of cancer. Uncovering its governing factors and underlying mechanism are important for cancer therapy. Here, we report that enhancer of zeste homologue 2 (EZH2), previously known as a transcription repressor and lysine...