However, the importance of F. necrophorum as a cause of simple sore throat in the community is unknown. Using quantitative real-time PCR with primers targeting the rpoB gene, 100 routine throat swabs collected from patients presenting to general practitioners with pharyngitis were analysed for the...
The primers were designed based on previous published sequences of closely related pines (Zhou et al., 2014). PCR and sequencing information have been described in detail in Zhou et al. (2014). We only used intron sequences in order to reduce the confounding effect of natural selection. ...
What is the role of RNA polymerase in protein synthesis? Why is Taq DNA polymerase used for PCR? Why does DNA polymerase require a primer? What is the purpose of DNA helicase? What is the role of DNA helicase during DNA replication?
Microorganisms often respond to their environment by growing as densely packed communities in biofilms, flocs or granules. One major advantage of life in these aggregates is the retention of its community in an ecosystem despite flowing water. We describe here a novel type of granule dominated by ...
PCRqPCRhigh-throughput sequencingprimer designbiodiversityEnvironmental DNA (eDNA) comprises DNA fragments that have been shed into the environment by organisms, and which can be extracted from environmental samples such as water or soil. Characterization of eDNA can allow researchers to infer the ...
5S rRNA is an indispensable component of cytoplasmic ribosomes in all species. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whether 5S rRNA autonomy i...
The abundances of the total bacterial community were estimated by real-time quantitative PCR (qPCR) assays. For each treatment, we used five equimolar mixtures prepared from pairs of the 10 DNA replicates extracts. The total bacterial community was quantified using 16S rRNA primers as described by...
What is the function of DNA ligase in recombinant technology? Why are nucleic acids important? Why is gel electrophoresis important? Why do molecular biologists primarily focus on B-DNA? What is the role of enzymes in DNA replication? Why are primers needed for DNA replication? How does RAD51...
Extracted DNA was amplified by PCR and submitted to high- throughput sequencing to evaluate SHM. As SHM in light chains is not under the 3'RR control9, IgH SHM values along rearranged JH4 sequences frequencies of were 1.45% normalised and 0.07% to Jk5 SHM were found in values. wt and ...
Copy numbers of TREC and KREC were determined using Quantitative Real-Time PCR assay performed on the 7500 Fast Real-Time PCR (Applied Biosystems). Primers and probes of TREC, KREC, and the reference gene TRAC were designed according to Sottini et al. (2010). TaqMan Universal master mix (...