Sequencing & analysis IDT ALIGN℠Program Method Data Figure 1. Normalase technology enables consistent pooling of RNA-seq libraries without compromising transcriptomic data quality.The IDT xGen RNA Library Prep Kit and the xGen Broad-Range RNA Library Prep Kit were used to create whole transcriptome...
Total RNA Sequencing Workflow Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted next-generation sequencing (NGS) technology, producing approximately 90% of global sequencing data.* In addition to high-quality data, Illumina offers integrated whole-transcriptome sequencing workflows...
TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling path- ways in hepatocellular carcinoma. Front Med. 2015;9:322-30.Ho DW, Kai AK and Ng IO (2015) TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways in ...
Whole Transcriptome SequencingBrief Fields Process Parameters Requirements Brief Whole transcriptome refers to the sum of all transcript information transcribed by a specific tissue or cell in a specific state, including mRNA, small RNA, lncRNA, circRNA and other coding and non-coding RNA. Four kinds...
The present study demonstrated the potential use of a nextgeneration sequencingbased cancer gene signature in patients with ZMnegative sGBM, indicating possible clinical therapeutic strategies for further treatment of such patients.ChenWangYuZhangLi
Utilizing bioinformatics methods, the present research analyzed the TCGA-BRCA dataset, which included four groups of transcriptome sequencing data as follows, normal (WT), NLRP3 knockout (KO), non-knockout-BRCA (BC-WT), and NLRP3-knockout-BRCA (BC-KO). Results indicated that NLRP3 was ...
(ceRNA) regulatory networks. We performed whole-transcriptome sequencing of 12 samples from four periods (Bud, leaves not expanded; Alb, albino leaves; Med, re-greening leaves; and Gre, green leaves) and identified a total of 6325 differentially expressed mRNAs (DEmRNAs), 667 differentially ...
Whole transcriptome sequencing identifies differential gene expression between normal and aged, degenerating stallion testesIn spite of the importance of sperm motility to fertility in the stallion, little is known about the signaling pathways that regulate motility in this species. In other mammals, ...
Whole transcriptome sequencing reveals genes involved in plastid/chloroplast division and development are regulated by the HP1/DDB1 at an early stage of to... Whole transcriptome sequencing reveals genes involved in plastid/chloroplast division and development are regulated by the HP1/DDB1 at an ...
We performed whole-transcriptome sequencing using RNA-Seq technology to construct transcriptome patterns of messenger RNAs (mRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs). These patterns were then subjected to in-depth analysis using bioinformatics tools.#We...