Total RNA Sequencing Workflow Illumina sequencing by synthesis (SBS) chemistry is the most widely adopted next-generation sequencing (NGS) technology, producing approximately 90% of global sequencing data.* In addition to high-quality data, Illumina offers integrated whole-transcriptome sequencing workflows...
Automation-friendly RNA-seq workflow What is whole transcriptome sequencing? RNA sequencing (RNA-seq) is a next generation sequencing method for analyzing the transcriptome of cells and assessing gene expression variation. ThexGen RNA Library Prep Kitand thexGen Broad-Range RNA Library Prep Kitare fa...
The bioinformatics workflow for WGS is similar to that for whole exome sequencing. You can view our articleBioinformatics Workflow for Whole Exome Sequencing. The bioinformatics workflow for WGS falls into the following steps: (1) raw read quality control; (2) data preprocessing; (3) alignment;...
The straightforward workflow–similar to PCR–allows someone with little to no next-generation sequencing experience to successfully perform the protocol.”–Richard Allcock, Director, Lotterywest State Biomedical Facility, Genomics School of Pathology and Laboratory Me...
Consequently, accurate whole-transcriptome amplification is required to analyze the transcriptome of a single cell by NGS. This unit describes the entire workflow from cells to NGS, including cell quality testing, cell lysis, gDNA removal, whole-transcriptome amplification, and NGS library preparation....
where it was suitable for sequencing of smaller selected parts of the genome, to WGS that became possible around 2010 and was FDA approved in 2018. The laboratory procedures are relatively simple and can be performed in any conventional molecular biology laboratory. The general WGS workflow is out...
Small insertion/deletions (InDels) and SNVs were detected using the GATK workflow (https://github.com/gatk-workflows/broad-prod-wgs-germline-snps-indels)16. Briefly, gVCF files were generated using GATK HaplotypeCaller via the “-ERC GVCF” option. To produce the final multisample VCF, all gV...
For every workflow, default analysis settings and parameters were used. The same reference transcriptome was used for all workflows (Ensembl GRCh37, release 75). For Tophat-Cufflinks and Tophat-HTSeq, the transcriptome was filtered for transcripts detected by the RT-qPCR assays prior to running ...
Establishment of a whole transcriptome data analysis workflow and cognate modules for quality control and quantitation using the SOLiD™ 3 systemUltra high-throughput sequencing of RNA (RNA-Seq) has shown great promise for the measurement ofwhole transcriptional activity at a single nucleotide ...
Here we try to demystify how to choose between real-time PCR (qPCR) and next-generation sequencing (NGS), or whether you really need to choose at all. Looking to commercialize?Contact our OEM services team today › Real-time PCR and whole transcriptome NGS ...