二、实验原理 基因组测序常用的两种策略是克隆法(clone-based strategy)和全基因组鸟枪法(whole genome shotgun method…wenku.baidu.com|基于1 个网页 2. 用全基因散弹法 ...碳糖六碳糖代谢途径中参与的基因,全基因序列定序我们用全基因散弹法(whole genome shotgun method),经过次世代定序仪GS…ntour.ntou....
Marker development using SLAF-seq and whole- genome shotgun strategy to finemap the semi-dwarf gene ari-e in barley. BMC Genomics. 2016;17:911.Jia Q, Tan C, Wang J, Zhang XQ, Zhu J, Luo H, Yang J, Westcott S, Broughton S, Moody D, Li C. Marker development using SLAF-seq and...
We sequenced the genome of a male GSM using a whole-genome shotgun strategy. Cleaned-up data provided 146-fold average coverage across 3.05 Gb of assembled sequence (Supplementary Fig. 2 and Supplementary Tables 1 and 2), with N50 values of 25.5 kb and 1.55 Mb, respectively, for contigs ...
A whole-genome shotgun strategy was used to sequence and assemble the genome of L. sativa cultivar Salinas from Illumina short reads. A total of 198.5 Gb Illumina paired-end and mate-pair reads were generated from seven libraries of different fragment sizes (170 bp to 40 kb; Supplementary ...
heimuer strain Dai 13782 using a whole-genome shotgun strategy by high-throughput Illumina HiSeq 4000 sequencing and the PacBio RSII long-read sequencing platform at BGI-Shenzhen, China. Paired-end reads were generated by sequencing of insert libraries of 350 bp and 10 kb using Illumina HiSeq...
Shotgun sequencing is a classic strategy for whole genome sequencing. The shotgun sequencing strategy provides a technical guarantee for large-scale sequencing. The technology first randomly interrupts a complete target sequence into small fragments, sequenced separately, and then splicing them into a ...
Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencingArticle Published: 01 December 2013 Volume 31, pages 1111–1118, (2013) Cite this article From View current issue Submit your manuscript Siddarth Selvaraj, Jesse R Dixon, Vikas Bansal & Bing Ren 17k Accesses 188...
In a shotgun metagenomic sequencing experiment, libraries were constructed using the xGen DNA Library EZ Kit and 1 ng of DNA derived from a mock metagenome DNA standard (ATCC, MSA-1000™) with varying GC content (30–68.8%). The kit provided comprehensive normalized coverage across di...
The bovine genome sequencing consortium sequenced a single inbred female Hereford cow and her sire using a combination of hierarchical sequencing and whole-genome shotgun sequencing [2]; the data have been assembled into two reference genomes, Btau and UMD [3, 4]. After the bovine reference ...
itinerans collected from Baoting County on Hainan Island and transplanted in South China Botanical Garden was sequenced by whole-genome shotgun strategy on the Illumina Hiseq2000 platform (Supplementary Note 1, Supplementary Fig. 1). A total of 74.2 Gb clean reads (approximately 120.7 fold ...