Whole genome sequencing (WGS) of Mycobacterium tuberculosis has rapidly progressed from a research tool to a clinical application for the diagnosis and management of tuberculosis and in public health surveillance. This development has been facilitated by drastic drops in cost, advances in technology and...
Whole genome sequencing (WGS) is the comprehensive read and analysis of an entire genome, including non-coding regions of the genome.
Whole genome sequencing can also help in the detection of antimicrobial resistance mechanisms and epidemiologic tracking and surveillance of pathogenic bacteria.Mustafa, Abu SalimMedical Principles & Practice
which uses custom designed 120-mer RNA oligonucleotides that span the entire genome, can recover (by hybridisation) low copy number herpesviruses from clinical samples with sufficiently high sensitivity and specificity to enable ultra-deep whole genome sequencing [19],[20]. In this study ...
The Darwinist explanation of development of species through time has often been referred to as “survival of the fittest.” One very important, present development in dangerous bacteria surrounding us is the creation of antibiotic resistant bacteria, i.e., bacteria that can withstand the antibiotics...
With next generation whole-genome sequencing, researchers and the food industry can “read” the few million nucleotides in suspect bacteria (or few thousand viral nucleotides) for around $100 and in just a few hours. In this way, they can quickly map differences and similarities between indivi...
tuberculosis genome has yet to be reported. 5. Sequencing from cultured bacteria DNA extracted from cultured bacteria may be used in genome-wide association studies (GWAS) to identify putative resistance mutations. In addition, isolation and culturing of the bacteria from sputum samples serves as an...
NUCLEOTIDE sequencingCOST effectivenessCITRUS greening diseaseMETAGENOMICSHuanglongbing (HLB) is a worldwide deadly citrus disease caused by the phloem-limited bacteria 'Candidatus Liberibacter asiaticus' (CLas) vectored by Asian citrus psyllids. In order to effectively manage this disease, it is crucial ...
It is also reported that only around 60% of cecum bacteria are culturable [17,18]. Some advanced techniques like temporal temperature gradient gel electrophoresis (TTGE), gradient gel electrophoresis (DGGE), terminal-restriction fragment length polymorphism (T-RFLP), and Sanger sequencing, were ...
Genomic sequencing is the most powerful approach for evaluating such microbial evolution. Next-generation sequencing technologies, including Illumina, PacBio, and 454 sequencing, have increased the speed and decreased the cost of genome-wide sequencing. Chin et al. sequenced two V. cholerae isolates ...