全外显子测序(Whole Exome Sequencing, WES):通过目标区域获取技术对外显子区域的DNA进行测序,可获取基因编码区域的核酸突变信息,从而研究其与疾病和其它生物性状相关性。相对于全基因组测序,全外显子可显著降低成本。 技术流程 技术参数 测序平台与方式:HiSeq, PE150捕获平台:Agilent SureSelect测序深度:100X,200X...
Whole exome sequencing works by using capture probes (synthesized oligonucleotides) or baits designed to specifically hybridize the exons (coding portions of genes), rather than enriching all the genomic DNA. Library preparation comes next, wherein sequencing adapters are added to the enriched protein-...
With the fastest run times of any benchtop next-generation sequencer, at the most affordable price, the Ion Proton Sequencer provides a simple, scalable, and affordable solution for whole exome sequencing in cancer research. Combined with theIon Chef Systemfor automated template prepar...
Exome sequencing is a targeted sequencing approach that is restricted to the protein-coding regions of genomes. The exome is estimated to encompass approximately 1% of the genome, yet contains approximately 85% of disease-causing mutations [1]. For genetic re...
About Whole Exome Sequencing The exome, comprising about 30 million base pairs and representing 22,000 genes, makes up roughly 2% of the genome. It is estimated that approximately 85% of disease-causing variants can be found within these protein-coding regions. WES therefore allows for a focus...
Whole exome sequencing (WES)doi:10.1002/9783527678679.dg14326This article has no abstract.Wiley‐VCH Verlag GmbH & Co. KGaAThe Dictionary of Genomics, Transcriptomics and Proteomics
The laboratory mouse was domesticated from the wild house mouse. Understanding the genetics underlying domestication in laboratory mice, especially in the widely used classical inbred mice, is vital for studies using mouse models. However, the genetic me
Whole exome sequencing (WES) is a targeted next-generation sequencing method that identifies all protein-coding genes (exons) in the genome. These regions contain most large genetic variants and single nucleotide polymorphisms (SNPs) associated with human disease. By enriching for exons, you can fo...
Whole-exome sequencing uncovers frequent GNAS mutations in intraductal papillary mucinous neoplasms of the pancreas. We performed whole-exome sequencing for a primary IPMN tissue, which uncovered somatic mutations in KCNF1, DYNC1H1, PGCP, STAB1, PTPRM, PRPF8, RNASE... T Furukawa,Y Kuboki,E ...
It is of note that we have previously used WES to search for low-frequency and rare variants for DKD in individuals with type 1 diabetes [11, 12]. A recent exome sequencing of >170,000 individuals identified rare coding variants in 35 genes for total cholesterol, LDLC, HDLC, triglycerides...