Whole blood RNA sequencing reveals a differential transcriptomic profile associated with cervical insufficiency: a pilot studyAlpha defensinCervical insufficiencyPregnancyPreterm birthRNA-SeqBackground The uterine cervix is a mechanical and immunological barrier against ascending infection during pregnancy. Cervical...
Blood is a highly informative, easily accessible, and minimally invasive sample type routinely used for diagnostic purposes and in clinical research to study pathological and physiological conditions and the impact of therapies. Whole blood transcriptome sequencing is an RNA sequencing (RNA-Seq)-based ...
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Whole blood RNA genotyping revealed a 1704GϾC mutation that was confirmed by sequencing exon 7 from genomic DNA. This creates a missense mutation, W568C, previously re- ported to cause LQTS23 (Fig. 2). The second KCNH2 mutation was found in an 11-year-old boy who had fainted a ...
Although the advent of single-cell RNA sequencing has made it possible to measure gene expression of specific blood cell types, there remain financial and technical barriers to its widespread use. Hence, the most common methods of blood RNA profiling either investigate RNA extracted from all blood...
Single-cell RNA sequencing of blood antigen-presenting cells in severe COVID-19 reveals multi-process defects in antiviral immunity Article10 May 2021 The differential immune responses to COVID-19 in peripheral and lung revealed by single-cell RNA sequencing ...
suis and transcriptional profiles of whole blood, generated by RNA-Seq, were analyzed and compared to non-infected animals. RNA-Seq showed 55 differentially expressed (DE) genes in the M. suis infected pigs. Down-regulation of genes related to innate immunity (tlr8, chemokines, chemokines ...
Circulating cell-free DNA (cfDNA) is becoming an increasingly important research sample to investigate circulating tumor DNA (ctDNA) for investigation of many cancer types. Usingwhole-genome next-generation sequencing(NGS),...
Single-cell sampling with RNA-seq analysis plays an important role in reference laboratory; cytogenomic diagnosis for specimens on glass-slides or rare cells in circulating blood for tumor and geneti...
Prostate cancer: two whole-blood RNA expression signatures for aggressive CRPC.Comment on Lancet Oncol. 2012 Nov;13(11):1105-13. Lancet Oncol. 2012 Nov;13(11):1114-24.doi:10.1038/nrurol.2012.195ClyneMelanieNature Reviews Urology