Isoform sequencing (Iso-Seq) uses long-read technology to produce highly accurate full-length reads of mRNA transcripts. Visualization of individual mRNA molecules can reveal new details of transcript variation within understudied portions of mRNA, such as the 5′ untranslated region (UTR). ...
Using sequencing technology combining full- length RNA sequencing and single-nucleus RNA sequencing (snRNA-seq) in combination with ultrastructural analyses, our study revealed a cellular map of Decaisnea insignis fruit at the single-cell level and identified different cell types. Re...
[3] Roger Volden, Christopher Vollmers. Highly Multiplexed Single-Cell Full-Length cDNA Sequencing of human immune cells with 10X Genomics and R2C2. bioRxiv 2020.01.10.902361; doi: https://doi.org/10.1101/2020.01.10.902361 [4] Lebrigand,...
Cloning, sequencing and expression of a full-length cDNA copy of the M1 double-stranded RNA virus from the yeast, Saccharomyces cerevisiae. Strains of the budding yeast, Saccharomyces cerevisiae, may contain one or more cytoplasmic viruses with double-stranded RNA (dsRNA) genomes. The killer ph....
length mRNA sequencing. The Encode RNA-PET dataset captures the connections between 5′ and 3′ ends of transcripts. These data were used for the validation of the combinations of TSSs and PASs in the transcript structures identified by full-length mRNA sequencing in MCF-7 cells. However, it ...
根据官方Application note-Kinnex full-length RNA kit for isoform sequencing文件中提供的饱和度曲线的数据显示(图6),单个转录组数据达到10M(1000万条)HiFi reads时,可检出80%的已知转录本(isoform)。而当到达20M(2000万条)HiFi reads时,饱和度曲线整体趋于平缓,这表明增加测序深度会新增少量转录本(isoform)的检出...
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease res...
RNA-Seq of single cells has been limited by biases in transcript coverage and unknown technical variability. Ramsköld et al. describe a protocol to reproducibly recover full-length transcripts and use it to quantitatively analyze splice isoforms in si
RNA-Seq of single cells has been limited by biases in transcript coverage and unknown technical variability. Ramsköld et al. describe a protocol to reproducibly recover full-length transcripts and use it to quantitatively analyze splice isoforms in si
根据官方Application note-Kinnex full-length RNA kit for isoform sequencing文件中提供的饱和度曲线的数据显示(图6),单个转录组数据达到10M(1000万条)HiFi reads时,可检出80%的已知转录本(isoform)。而当到达20M(2000万条)HiFi reads时,饱和度曲线整体趋于平缓,这表明增加测序深度会新增少量转录本(isoform)的检出...