Four factors that determine "what makes a good nucleophile" are its charge, electronegativity, the solvent, and the steric bulk. Let's discuss each in turn.
Explain why quinuclidine is a much more reactive nucleophile than triethylamine, even though both compounds have N atoms surrounded by three R groups. Explain why there exists a correlation between electrostatic potential and acid strength. Does phenol...
After the strand passage event, the DNA is re-ligated in the reversible reaction, where the free 3′-OH group of the DNA acts as a nucleophile. For this transesterification reaction, divalent cations are required (usually magnesium), which are coordinated by conserved residues of the TOPRIM ...