qPCR/Real-Time PCR Instrumentation What Is Real-Time PCR? In conventional PCR, the amplified DNA product, or amplicon, is detected in an end-point analysis. In real-time PCR, the accumulation of amplification product is measured as the reaction progresses, in real time, with product quantificat...
Although qPCR is by far the most frequently used molecular technique in bacterial diagnostics, in certain scenarios a broad-range (non-specific) 16S rDNA (ribosomal DNA) PCR is increasingly being used. Broad-range 16S rDNA PCR is also more commonly used in research settings, originally for use...
The simplest, and most commonly used, type of multiplexing is duplexing, in which two genes are amplified in a single reaction. Typically, in a relative qPCR experiment designed to determine the fold change differences in gene expression, these will be a single gen...
qPCR qPCR – also called real-time PCR, quantitative PCR or quantitative real-time PCR – is a technique used to detect and measure the amplification of target DNA as it is produced. In contrast to conventional PCR reactions, qPCR requires a fluorescent intercalating dye or fluorescently-...
The qPCR assay is a mainstay of microbiological diagnostics within the National Health Service (NHS). At our hospital approximately 200 qPCRs are performed per week for the investigation of bacterial infections. Although qPCR is by far the most frequently used molecular technique in bacterial ...
What is the Cq Value? Real-time PCR(often called qPCR) is usually conducted to quantify the absolute amount of a target sequence or to compare relative amounts of a target sequence between samples. This technique monitors the amplification of the target in real time via a target-specific fluo...
To better understand the Ct value, it is worthwhile looking at the amplification plot of a qPCR run.Whilst performing a qPCR experiment, the results are commonly displayed on an amplification plot. An example of a typical amplification plot, along with annotations, can be seen below....
- US-based precision molecular diagnostics company DiaCarta Inc is launching a new suite of CE-IVD marked highly sensitive hydrolysis probe (TaqMan qPCR) based QClamp assays for detection of common cancer mutations in genes, such as KRAS, NRAS, BRAF, EGFR, PIK3CA and JAK2, using tissue biopsy...
Reverse transcriptase, also called RNA-directed DNA polymerase, an enzyme encoded from the genetic material of retroviruses that catalyzes the transcription of retrovirus RNA (ribonucleic acid) into DNA (deoxyribonucleic acid). This catalyzed transcription is the reverse process of normal cellular transcrip...
qPCR is typically used to determine the copy number of a DNA sequence of interest and to detect, characterize, and detect the presence of pathogens in a sample. RT-qPCR RT-qPCR refers to reverse transcription quantitative real-time PCR. This technique combines RT-PCR with ...