To understand how real-time PCR works, we illustrate a qPCR analysis using a typical amplification plot (Figure 1). In this plot, the number of PCR cycles is shown on the x-axis, and the fluorescence from the amplification reaction, which is proportional to the amount of amplified product ...
In multiplexing, you can reduce the amount of sample required for a qPCR reaction by measuring the expression of more than one gene in a reaction. The process is as sensitive and accurate as single-gene amplification (or singleplexing), but more technically comple...
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Although qPCR is by far the most frequently used molecular technique in bacterial diagnostics, in certain scenarios a broad-range (non-specific) 16S rDNA (ribosomal DNA) PCR is increasingly being used. Broad-range 16S rDNA PCR is also more commonly used in research settings, originally for use...
If you are performing quantitative PCR (qPCR), then it’s crucial you understand what a Ctvalue is. We’ll take you through its critical role in qPCR, its varying names, how it’s calculated, and what it means when it all seems to go a bit wrong. ...
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Quantitative polymerase chain reaction (qPCR) technology is also common, with machines combining fast detection and quantification with high-throughput capacity. Other applications for PCR testing Medical cannabis isn’t the only industry that relies heavily on PCR testing. Th...
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Reverse transcriptase, also called RNA-directed DNA polymerase, an enzyme encoded from the genetic material of retroviruses that catalyzes the transcription of retrovirus RNA (ribonucleic acid) into DNA (deoxyribonucleic acid). This catalyzed transcription is the reverse process of normal cellular transcrip...