qPCR Assay Design and Optimization Real-Time PCR Data Analysis What is High Resolution Melting (HRM)? Introduction to qPCR System MIQE and RDML Guidelines qPCR/Real-Time PCR Reagents Real-Time PCR Troubleshooting Oligonucleotides: Design and Applications Introduction to PCR Primer & Probe ChemistriesNuc...
qPCR qPCR – also called real-time PCR, quantitative PCR or quantitative real-time PCR – is a technique used to detect and measure the amplification of target DNA as it is produced. In contrast to conventional PCR reactions, qPCR requires a fluorescent intercalating dye or fluorescently-...
Multiplexing will not always be appropriate for your qPCR experiments. Before you embark on a multiplex experiment, optimize the assay conditions and validate them carefully. Confirm that results obtained from multiplexing are the same as results obtained from singleplex r...
The qPCR assay is a mainstay of microbiological diagnostics within the National Health Service (NHS). At our hospital approximately 200 qPCRs are performed per week for the investigation of bacterial infections. Although qPCR is by far the most frequently used molecular technique in bacterial ...
The qPCR assay is a mainstay of microbiological diagnostics within the National Health Service (NHS). At our hospital approximately 200 qPCRs are performed per week for the investigation of bacterial infections. Although qPCR is by far the most frequently used molecular technique in bacterial ...
The tests are carried out by the assay developed within their verified and validated laboratories. The turn-around time from sample collection to the result is one to two weeks. For some patients, even this short time period can be critical. Days, let alone weeks, feel like a long time ...
Genes identified in all types of transcriptomic analyses need to be further investigated using molecular approaches. RT-qPCR is the method of choice for evaluating the expression profiles of selected genes. Typically, at least three biological replicates are prepared to confirm differential transcript exp...
Confidence in your results is vital when people are counting on you. Detect your target 100% of the time with TaqMan® Assays and reagents. The combination of the most advanced assay design pipeline in the industry and the benefits of MGB probes delivers the specificity you need to detect ...
(and insoluble Aβ levels) has been validated using quantitative immunohistochemistry, western blotting, enzyme-linked immunosorbent assay (ELISA) and meso scale discovery (MSD) assays. Importantly, this reduction in amyloid deposition is sex-specific and only occurs in males in the APPPS1-21 and ...
What is PEA? Technical film Set up Olink in your lab Proximity Extension Assay A unique combination of specificity and scalability enables high-throughput, multiplex protein biomarker analysis. Application Application Disease areas Population-scale proteogenomics ...