For example, when staining cellular F-actin it is imperative to use a methanol-free formaldehyde fixative. This is because methanol can disrupt F-actin during the fixation process and prevent the binding of phalloidin conjugates. "Pure" methanol-free formaldehyde can be made by...
DAPI staining (blue fluorescence) highlights the nuclei of cells; phalloidin/rhodamine staining (green fluorescence) shows cytoskeletal components. Magnification: 63×; scale bar: 20 µm. 2.6. ROS Levels Evaluation The intracellular production of ROS was examined to confirm that the cultured ...
Twitter Google Share on Facebook Medical Wikipedia AcronymDefinition DAPI4',6-Diamidino-2-Phenylindole(double stranded DNA staining) DAPIDelaware Adolescent Program, Inc.(Wilmington, DE) DAPIDimensional Assessment of Personality Impairment(psychiatric screening) ...
In the supplemental material, we have done the whole-mount immunostaining with phalloidin (green), peripherin (red), and Ctbp2 (blue) in the apical (S1: A, D, and G), middle (S1: B, E, and H), and midbasal (S1: C, F, and I) turns of the cochlear in the surgically prepared...
[micro]M and incubated for 30 minutes prior to imaging; TMRM dye was added to live, unfixed HepaRG cells at a concentration of 0.1 [micro]M and incubated for 30 minutes prior to imaging; Alexa Fluor 568 Phalloidin was added to fixed HepaRG cells at a concentration of 0.165 [micro]M ...