Here we provide a detailed protocol for culturing primary rat cortical neurons, Phalloidin staining for F-actin puncta, and subsequent quantification techniques. First, the frontal cortex of E18 rat embryos are dissociated into low-density cell culture, then the neurons grown in vitro for at least...
The organization of F-actin in vegetative cells of the brown alga Sphacelaria rigidula was studied after staining with a modified rhodaminephalloidin (Rh-Ph) protocol. The interphase vegetative cells display a well-organized F-actin cytoskeleton, consisting of cortical, endoplasmic and perinuclear ...
as reported by others11, the visualization of the MPS by βII-spectrin was more clear and robust than by phalloidin staining, giving invariable superiorabundance
The binding assay in Fig. 3D was performed as above but with following modifications: F-actin, stabilized using phalloidin in a 1:1 molar ratio, was varied from 0 to 1.5 μ M. The ABD concentration was held constant at 0.8 μ M. This 0.8 μ M ABD concentration was...
Component A:iFluor 488-Phalloidin;Component B:Labeling Buffer;Protocol 公司简介 北京百奥莱博科技有限公司是一家专业的生物试剂销售公司。公司主要经营分子生物学试剂、细胞生物学试剂、血液制品、抗体抗原、食品安全检测试剂盒及各种生化检测试剂盒,可以为您提供专业的生物技术服务,以优良的价格、*的服务、优先的理念...
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?ActinPhalloidinF-actin Posted on April 2, 2019Do I need to deparaffinize my paraffin-embedded tissue sections before staining F-actin with Phalloidin-iFluor® conjugates? Can you provide a deparaffinization protocol?
To detect the protein–protein interactions in situ, a DuoLink in situ PLA kit (Merck) was used according to the manufacturer's protocol. After fixation and permeabilization, oocytes were incubated in blocking solution and immunolabeled (overnight, 4 °C) with primary antibodies diluted in the ...
Phalloidin stabilizes F-actin and accomplishes two things: (i) it reduces the amount of actin that does not sediment during centrifugation and (ii) it allows F-actin to be diluted below the critical concentration (~0.5 µM), which is often necessary if varying the amount of F-actin to ...
It is important to note that phalloidin staining to visualize F-actin cannot be used to investigate endothelial actin structures that are in close proximity of transmigrating leukocytes, since F-actin in both leukocytes and ECs are visualized by phalloidin staining, making it impossible to ...
Phalloidin staining in aged brains revealed that neuronal Fhos-RNAi induction abrogated the F-actin-rich rods observed in aged control brains (Fig. 2a, b). To deepen our understanding of F-actin dynamics in aging brains, and to determine if neuronal Fhos knockdown had universal effects through...