我们提供一些 InvitrogenMolecular Probes荧光和生物素化的鬼笔环肽衍生物,与肌动蛋白标记抗体相比具有明显优势。鬼笔环肽偶联物的结合特性不会随物种(包括动植物)的肌动蛋白变化而发生明显改变。此外,它们的非特异性染色可忽略不计,因此染色区和未染色区...
Introduction Actin is a globular, roughly 42-kDa protein found in almost all eukaryotic cells. It is also one of the most highly conserved proteins, differing by no more than 20% in species as diverse as algae and humans. Actin is the monomeric subunit of two types of filaments in cells:...
Actin staining is very useful in determining the structure and function of the cytoskeleton in living and fixed cells. The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures. ...
Actin staining is very useful in determining the structure and function of the cytoskeleton in living and fixed cells. The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures. ...
Rhodamine-phalloidin staining of F-actin in pollen after dimethylsulphoxide permeabilization[J] . Elisabeth S. Pierson.Sexual Plant Reproduction . 1988 (2)Pierson, E. S., Rhodamine-phalloidin staining of F-actin in pollen after dimethylsulphoxide permeabilization, Sex Plant Reprod., 1988, (1): ...
Bundles of actin filaments were observed in in vitro cultured pollen of Lilium longiflorum and Nicotiana tabacum which had been permeabilized in a buffered medium containing 5% dimethylsulphoxide (DMSO), EGTA, rhodaminephalloidin for F-actin staining, and sucrose as an osmoticum. In imbibed pollen ...
Rhodamine-phalloidin staining for F-actin was carried out on both blade and conchocelis phases of Porphyra leucosticta. Cells in the blade have a diversity... McDonald,Garbary,Mitman,... 被引量: 0发表: 1992年 Actin﹎ediated chloroplast movement in Griffithsia pacifica (Ceramiales, Rhodophyta...
This chapter describes the staining of actin with fluorochrome-conjugated phalloidin. Phalloidin interacts specifically with yeast and other actins; the interaction appears to be specific for polymerized rather than unpolymerized actin. The coupling of fluorochromes to phalloidin thus provides a quick an...
1. [Fluorescent staining of actin filaments in fixed tissue sections and tissue culture cells preparations]; 2.[Preparation of stabilized fluorescent actin filaments in vitro.] 肌动蛋白染色(Actin Staining)特别适用于确定活细胞和固定细胞内细胞骨架的结构和功能。活细胞内的肌动蛋白细胞骨架很有活力和结构不...
Immuno-staining/fluorescence Starting Material Primary Human cells Tips Carefully prepare the stock solution in methanol Results Summary Red color filaments like structures observed in fluorescence microscopy confirming the F-actin filaments. DOI or PMID # ...