What is the enzyme needed for a PCR reaction? a. RNA polymerase b. Taq polymerase c. DNA polymerase d. All the above Which of the following enzymes play an important role in DNA replication? (a) DNA helicase (b) DNA ligase (c) DNA primase (d) All of them (e) DNA polymerase III...
Polymerase chain reaction (PCR) is used to amplify specific DNA regions. What components are not needed for this reaction? (a) DNA primers (b) Taq polymerase (c) Deoxynucleotides (d) RNA primer. Why cannot the polymerase goes from 3' to 5'?
What is a PCR reaction, and why is it a useful technique? A person is setting up a PCR reaction and accidentally add twice as much of the salt buffer than what was supposed to. How will this impact product formation? What is the annealing step of a PCR ...
The real time PCR instrument used for performing amplification must be capable of distinguishing precisely between these fluorescent labels and measuring the signals produced by the amplification of each gene. Typically, the probe for your gene of interest is labeled with ...
ait is important that the stringency of the annealing step of the PCR reaction be somewhat permissive and not pushed beyond what is necessary to ensure a clean PCR product. 它是重要的PCR反应的焖火步的银根紧是有些许可和没在什么之外推挤是必要保证一个干净的PCR产品。[translate]...
4.What is the purpose of the positive and negative control tubes in your PCR amplification?What are the expected results for each? 5.Following the PCR amplification, what methods are commonly used to analyze the results? How can ...
Now that we know all the components required for PCR, let’s check out the threestepsinvolved in a PCR reaction. The steps are denaturation, annealing, and extension. The activity of the polymerase is dependent on the presence of single single-stranded DNA to which the primers can bind. T...
During PCR and cycle sequencing, the DNA is first denatured (the double-stranded DNA template becomes single-stranded DNA). A subsequent annealing step allows for hybridization of the oligonucleotide primer close to the sequence of interest. In the extension...
What is the enzyme needed for a PCR reaction? a. RNA polymerase b. Taq polymerase c. DNA polymerase d. All the above A researcher mistakenly uses a heat-sensitive version of DNA polymerase in a PCR. Which of the following will occur? ...
What is the role of the beta-prime part of RNA polymerase? How does RNA polymerase generate RNA without a primer? What is the function of RNA polymerase II? Where is it found? Define the following: RNA polymerase. What is the enzyme needed for a PCR reaction? a. RNA polymerase b. Taq...