aberrant gene expression resulting from disrupted HAT and HDAC activities can alter immune responses or neuronal signaling, leading to disease. Thus, the role of histone acetylation in regulating gene expression is critical across a wide spectrum of biological...
histoneepigeneticThe CENP-A histone H3-like variants are centromere-specific histones found in all eukaryotes examined to date, from budding yeast to man. New experiments using antibodies, green fluorescent protein fusions, and epitope tags show that CENP-A replaces the major histone H3 subunits in...
What is the biochemical piece of DNA? Below is the sequence of bases in eukaryotic DNA. (a) What is the complementary DNA sequence; and (b) the amino acids sequence of the protein? 5'-TTAAATGCCCTTGGATTCTTGA-3' For some reason, a mutation has occurred in the above DNA sequence, of ...
For a ChIP assay, chromatin is extracted, sheared and then added into a microwell where protein-DNA complexes are captured by affinity antibodies. The captured DNA is then released and purified. Eluted DNA can be used for various downstream applications including ChIP-PCR, ChIP-on-chip, and ChI...
et al. Dietary fiber-induced improvement in glucose metabolism is associated with increased abundance of Prevotella. Cell Metab. 22, 971–982 (2015). This work exemplifies that the presence of a specific gut microbiota composition in humans can dictate the host response to food. Article CAS Pub...
What is the part of the cell cycle in which DNA is synthesized? What does chromatin remodeling mean? What are the constituents of DNA and RNA? How is RNA formed from DNA? What is a histone Octamer made of? What is the nucleosome is made of?
TIN2 is a tankyrase 1 PARP modulator in the TRF1 telomere length control complex. Nat. Genet. 36, 618–623 (2004). CAS PubMed Google Scholar Ye, J. Z. et al. POT1-interacting protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex. Genes Dev. 18...
After cleavage, a stretch of 100 to 250 adenine residues is added to the 3’-hydroxyl group. RNA splicing removes introns before the transportation of completed mRNA to the cytoplasm. In animal cells, all mRNAs, except histone mRNAs, have a 3’-poly-A tail. The poly-A-binding protein ...
Prior to AFDB, teams of scientists would spend months, even years, solving a structure of a single protein, whereas nowadays, thanks to AFDB, protein structure is often taken as a given. However, we and others [Citation26] advise caution given that, although informative, all structural models...
Being this the case, one may wonder what set of physicochemical, structural and thermodynamic constrains need to be satisfied if a protein is to become a transcription factor, and moreover a master regulator? Some hints will be discussed.