sequence is present [1,2]. Cas9 nucleases with alternative PAMs have also been characterized and successfully used for genome editing [3]. It is important to note that the PAM is not present in the crRNA sequence but needs to be immediately downstream of the target site in the genomic DNA...
The protospacer adjacent motif (PAM) is a short DNA sequence (usually 2-6 base pairs in length) that is about 3-4 nucleotides downstream from the DNA sequence targeted by Cas9. Cas9 cannot bind to or cleave the target DNA sequence if it is not followed by the PAM sequence...
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Das, "CRISPR-Cas9 can inhibit HIV-1 replication but NHEJ repair facilitates virus escape," Molecular Therapy, vol. CRISPR/Cas9 System: A Bacterial Tailor for Genomic Engineering Lastly, the CRISPR-Cpf1 system identified in Acidaminococcus and Lachnospiraceae bacteria, which requires the PAM motif ...
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