CRISPR/Cas9xCas9corazoninmigratory locustcuticleenvironmental adaptationCRISPR/Cas9-based gene editing research has advanced greatly and shows broad potential for practical application in life sciences, but the
In addition, the RuvC domain used by AceCas9 is the sole catalytic domain of another commonly used type of CRISPR endonucleases, Cas12, such as Cpf1 (ref. 19). AceCas9 is moderately thermophilic and specific to DNA protospacers associated with a 5′-NNNCC-3′ PAM. Although the wild-...
DNA damage response (DDR) deficiency has been one of the emerging targets in treating breast cancer in recent years. On the one hand, DDR coordinates cell cycle and signal transduction, whose dysfunction may lead to cell apoptosis, genomic instability, and tumor development. Conversely, DDR defici...
Uncontrollable cancer cell growth is characterized by the maintenance of cellular homeostasis through the continuous accumulation of misfolded proteins and
In a new approach, we can use nanocarriers for clustered regularly interspaced short palindromic repeats (CRISPR) which is a high-precision and possible genome editing tool to be used in gene therapy. Tanaka et al. showed a viewpoint for the treatment of COVID-19 from the CRISPR/Cas9 system...
www.nature.com/scientificreports OPEN The role of PemIK (PemK/PemI) type II TA system from Klebsiella pneumoniae clinical strains in lytic phage infection Ines Bleriot1,7, Lucia Blasco1,7, Olga Pacios1,7, Laura Fernández‑García1,7, Antón Ambroa1,7, María ...
PKC is frequently mutated in human cancers. To uncover whether loss or gain of PKC function contributes to cancer progression, we selected mutations throughout the primary sequence and family membership and assessed their functional impact. Specifically, we asked how these cancer-associated mutations al...
2.2. Sequence retrieval and phylogenetic analysis The amino acid sequence of ZmFIE1 was searched for putative homologs in Ensemble Plants (https://plants.ensembl.org/index.html). Retrieved sequences were used to construct a phylogenetic tree using the neighbor-joining method in MEGAX [38]. MCSca...
Extended Data Figure 1 Validation of CRISPR–Cas9-mediated gene targeting. a, b, Gene-targeting strategy for Trp53 (a) and Smad4 (b). The protospacer adjacent motif (PAM) is underlined on the target sequence and the allelic fraction of each haplotype is indicated. c, d, Sequence alignment...
However, the systems so far proposed were based on artificial 3′UTRs containing multiple repeated perfect complementarity sequences or miRNA binding sites, instead of naturally occurring 3′UTRs, and generally lacked essential non-binding mutant controls [23]. To develop a robust and controlled ...