REAGENT or RESOURCESOURCEIDENTIFIER Antibodies Chicken Anti-Cas1 Shiimori et al., 2017 N/A Chicken Anti-Cas2 Shiimori et al., 2017 N/A Chicken Anti-Cas4-1 Shiimori et al., 2017 N/A Chicken Anti-Cas4-2 This paper N/A Chicken Anti-Csa2 Majumdar et al., 2015 N/A HRP Donkey Anti...
Derived from the CRISPR-Cas systems of prokaryotes1,CRISPR-associated (Cas) proteins operate in complex with their RNA guides to license sequence-specific nucleic acid targeting. The sequence specificity of Cas nucleases that are commonly used for DNA editing, includingStreptococcus pyogenesCas9 (SpCas9...
Bottom: Nme2Cas9 inhibition mirrors that of Nme1Cas9, except for the lack of inhibition by AcrIIC5Smu. (B) Genome editing in the presence of the five previously described anti-CRISPR families. Plasmids expressing Nme2Cas9 (200 ng), sgRNA (100 ng), and each respective Acr (200 ng) were...