● 表达CD73、CD90和CD105表面抗原; ● 不表达各种造血细胞表面标志分子(例如CD45、CD34、CD14或 CD11b、CD79a或CD19)和人类白细胞DR抗原(HLA-DR); ● 具有多向分化能力,可以在体外诱导分化成多种细胞,例如成骨细胞、脂肪细胞和成软骨细胞。 该研究分析了不同贴壁细胞解离方法对滑膜间充质干细胞细胞活力及表...
Jun 05, 2024 - Mesenchymal stem cells (MSCs) are adult stem cells with the ability to differentiate into various types of mesoderm-derived cells, showing promising results in preclinical studies for various medical conditions. Research continues to explo
Second, MSCs must express CD105, CD73 and CD90, and MSCs must lack expression of CD45, CD34, CD14 or CD11b, CD79伪 or CD19 and HLA-DR surface molecules. Third, MSCs must differentiate into osteoblasts, adipocytes and chondroblasts in vitro. MSCs are isolated from many adult tissues, ...
High levels of GM-CSF are produced by CD14+ CD16+ HLA-DHRlow inflammatory monocytes that express high levels of IL-6 and can infiltrate the lungs by amplifying inflammation and potentiating alveolar damage. Dendritic cells (DCs) are crucial in orchestrating both innate and adaptive immune ...
TRPA1 expression in primary sensory neurons (A), vascular endothelial cells (B), synoviocytes (C), inner ear hair cells (D), enterochromaffin cells, epithelial cells (E), keratinocytes, melanocytes, Schwann cells, mast cells (F), dental pulp and gingiva fibroblasts (G), pancreatic β-cells ...
To determine the lineage of specific differentiation antigens, cells were incubated with 1 [micro]L of R-phycoerythrin (RPE)-conjugated monoclonal antibody: CD3-RPE or Ig[G.sub.1] isotype-RPE for T lymphocytes and CD14-RPE or Ig[G.sub.2a] isotype-RPE for monocytes (all from Dako). Flow...
Immunosenescence of Natural Killer Cells, Inflammation, and Alzheimer's Disease Next, we identified the three main monocyte subpopulations in a dot plot of CD14 versus CD16: [CD14.sup.++] [CD16.sup.-], [CD14.sup.++][CD16.sup.+], and [CD14.sup.+][CD16.sup.++]. Circulatory Immune...
Fig. 1: Mechanism of regulatory T cells. Tregs down-regulate the expression of CD80/CD86 in DCs through CTLA-4, increase the expression of PD-L1, promote the binding of Treg-DC, reduce the secretion of IL-12, and hinder the antigen presentation.Tregs express IL-2 receptor, which competiti...
This review encompasses the following sections: A) Aging and Stem Cells in General, B) Age-Related Changes in HSCs/HPCs and Hematopoiesis, C) Age-Related Clonal Hematopoiesis of Indeterminant Potential (CHIP) and Inflammation, D) DNA Damage, Transcriptional and Epigenetic Changes During Aging, E...
Under inflammatory conditions, CD11b+ subsets of DCs also include a population of monocyte-derived cells, which might also express PD-L2 and CD301b (Gao et al., 2013). In this issue of Immunity, two complementary studies explore the role of specific DC subsets in Th2 cell-mediated ...