A plasmid is usually a circular (sometime linear), double strandad DNA, that can replicate itself autonomously. These are found in the cytoplasm of the bacterial cell, plasmids normally remain separated from the chromosome, but sometimes may integrate in
Why do plasmids use to carry foreign genes into E. coli cells invariably also carry the gene for resistance to an antibiotic? a. The gene is required for cellular replication. b. The gene is necessary for the lac operon system to operate. c. The gen Plasmids are often used as c...
Which plasmids are present in the bacterial cells? What is the evidence of conjugation in bacteria? What is used to treat bacterial infections in humans? What are the differences between bacteria and archea? What are the four phases of the bacterial growth curve?
Platt RM, Geesey GG, Davis JD, White DC (1985) Isolation and partial chemical analysis of firmly bound exopolysaccharide from adherent cells of a freshwater sediment bacterium. Can J Microbiol 31: 675–680 Article CAS Google Scholar Priest FG (1992) Enzymes, extracellular. In: Lederberg J (...
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1 Recombinant viral particles used for transduction are commonly produced by transfecting mammalian cells with specialized plasmids. See Figure 3 for the transduction pathway of adeno-associated virus (AAV), a ssDNA virus commonly used for transductions, in a mammalian cell. To perform a transduction...
An advantage of a high plasmid copy number is the greater stability of the plasmid when random partitioning (i.e., partitioning of plasmids into daughter cells) occurs at cell division. However, a high number of plasmids can also result in lower yields. Let’s look at a few cases where...
Step-by-Step Solution:Step 1: Define Recombination Recombination is the biological process that results in the exchange of genetic material between different DNA molecules, leading to the formation of new combi
Do prokaryotic cells have RNA? In eukaryotes, describe what genes each RNA polymerase transcribes. In E.coli DNA replication, what is the enzyme that adds nucleotides to the growing daughter strand? Is the RNA and DNA polymerase in prokaryotes and eukaryotes the same? If not what are the dif...
What features are important for a plasmid that is used in recombinant DNA procedures? After transforming E.coli with the plasmids, how can we identify the cells that contain a chimeric plasmid (containing DNA insert)? Describe the structure of a T-even bacteriophage. Describe the shape of RNA...