Plasmidsare autonomously replicating DNA molecules present in microorganisms. Plasmids are also known to bemobile genetic elementsthat can be horizontally transferred among different organisms [1,2]. Plasmids can be considered asgeneticparasites in the sense that their reproduction depends to some extent ...
Direct visualization of plasmid DNA in bacterial cells. Mol Microbiol. 1992 Jan; 6 (2):165–170. [ PubMed ]Eliasson,A., Bernander,R., Dasgupta,S. and Nordstrom,K. (1992) Direct visualization of plasmid DNA in bacterial cells. Mol. Microbiol., 6, 165±170....
In native cells plasmids are present in multiple forms (supercoiled, open circle, linearized, monomers, dimers and multimers) and appearing of several bands on a gel can be a reason for problems in their accurate quantification. To overcome this problem, restriction endonucleases can be used to ...
This is the modified alkaline lysis portion of the protocol, in which cells are split open using a combination of detergent and high pH. Sign in to download full-size image Figure 11.15. Location of DNA in the bacterial cell. The chromosome, which is attached to the inner cell membrane, ...
Plasmid functions can benefit their bacterial hosts, nevertheless, plasmid replication and gene expression utilizing the host machinery are considered a metabolic burden6. Consequently, in the absence of selection for plasmid-encoded traits, plasmid-free cells outcompete plasmid-carrying cells, which leads...
our results may help to explain the high prevalence of plasmids in nature. The results also indicate that the threshold conjugation rate for plasmid persistence may be lower than previously thought. In fact, once plasmids are present in multiple members of a community, they may be able to persi...
Not all plasmids can transfer themselves, but non-conjugative plasmids can be mobilized by other conjugative plasmids present in the same donor cell. Apart from this optional transfer ability, all bacterial plasmids contain the basic genetic information necessary for self-replication and segregation into...
We present the first examples of thermotropic biaxial mesophases in mixtures of model attractive uniaxial molecules. Nematic and smectic biaxial phases are... A Cuetos,A Galindo,G Jackson - 《Physical Review Letters》 被引量: 36发表: 2008年 Liquid crystalline polycarbosilanes and poly(di- n -...
The first step in the invention of binary vectors was the discovery thatvirgenes are not present in the T-DNA itself (De Greve et al., 1981;Garfinkel and Nester, 1980;Holsters et al., 1980;Koekman et al., 1979;Leemans et al., 1981) implying that thevirgenes are only involved in T...
Bacterial cells are useful as host cells suitable for the nucleic acid molecule and/or plasmid as described herein. In various embodiments, the host cell can comprise any various strain ofE. coli(e.g., JM110, HB101, DH5, DH10, and MC1061). In embodiments, cells comprising the nucleic ...