Download PDF Green fluorescent protein, more commonly known as GFP, was discovered in 1962. Since then, it has become one of the most widely exploited proteins for scientific research and …Read More » Selecting a Secondary Antibody for Bead-Based Applications ...
westernblot原理和常见故障分析-绿谷生物网 http://.ibioo/experiment/protein/western/2008/3150.html[2011/7/1112:41:00] 关于westernblot原理和常见故障分析原理:通过电泳区分不同的组分,并转移至固相支持物,通过特异性试剂(抗体)作为探针,对靶物质进行检测,蛋白质的Western印迹技术结合了凝胶电泳的高分辨率和固相...
Overcome your Western blot difficulties with our troubleshooting advice, covering problems such as weak/no signal, non-specific bands, high signal, and other common issues.
Tackle high background staining on your western blot. Antibody concentration too high, Insufficient blocking, Inadequate washing and more.
Western Blot Troubleshooting: Why Does The Observed Protein Molecular Weight (MW) Differ From The Calculated One? Western Blot Troubleshooting: High Background Western Blot Troubleshooting: Weak/No Signal & Other Western Blot ppt Western Blot Video Protocol...
3) Switch to a different blocking reagent and/or block the blot for less time. Using the same solution of diluted primary antibody repeatedly Use freshly-diluted antibody for each western blot because the...
HandbookandTroubleshootingGuide FeaturingtheSuperSignal ® WestFamilyofProducts Formoreproductinformation,ortodownloadaproduct instructionbooklet,visit.piercenet. TableofContents Introduction...1 WesternBlottingOverview...2-3 TransferProteintoaMembrane...4 MemCode™...
ReBlot™ Plus Western Blot Recycling Kit 56 5 Protein Digestion Protocol 57 5.1 On-Membrane Protein Digestion for Mass Spectrometry 57 6 Blot Storage Protocol 58 6.1 Preparation of Blots for Long-Term Storage 58 6 Appendices 59 Troubleshooting 65 Glossary 66 References 67 Patents 69 7 Ordering ...
Western Blot Troubleshooting Guide Common problems encountered in western blotting include low signal or high background. This video helps you troubleshoot problems in your Western Blot experiment, and provides solutions to ensure you get the expected results in the shortest amount of time. Read more...
Western Blot Antibody Dilution Calculator In this western blot troubleshooting section, we will help you visually identify specific and common problems on your western blots, such as high background, weak or no signal, multiple bands, uneven sta...