Tackle high background staining on your western blot. Antibody concentration too high, Insufficient blocking, Inadequate washing and more.
Overcome your Western blot difficulties with our troubleshooting advice, covering problems such as weak/no signal, non-specific bands, high signal, and other common issues.
Western Blot Antibody Dilution Calculator In this western blot troubleshooting section, we will help you visually identify specific and common problems on your western blots, such as high background, weak or no signal, multiple bands, uneven sta...
If the background remains high, but with strong band intensity, decrease the concentration of the secondary antibody. Concentrated primary antibody used Decrease the concentration of the primary antibody. How c...
Western Blot Troubleshooting Guide Common problems encountered in western blotting include low signal or high background. This video helps you troubleshoot problems in your Western Blot experiment, and provides solutions to ensure you get the expected results in the shortest amount of time. Read more...
Western Blot Troubleshooting: Why Does The Observed Protein Molecular Weight (MW) Differ From The Calculated One? Western Blot Troubleshooting: High Background Western Blot Troubleshooting: Weak/No Signal & Other Western Blot ppt Western Blot Video Protocol...
Figure 1: Western blotting after IP – Anti-Light Chain specific antibodies to avoid obscuring analytes in the 50 kDa range. Gels were loaded with Mouse IgG, whole molecule (015-000-003) After SDS-PAGE and transfer to nitrocellulose, blots were blocked with BSA (10% w/v). After incubation...
多用于Western Blot封闭阶段，此外实验条件允许的情况下还可用于Wetern Blot过程中抗体（一抗、二抗）的...
Transfer of high molecular weight proteins using the iBlot 2 Gel Transfer Device wb实验中没有信号条带可能的原因? 1)样本里是否含有目标蛋白;目标蛋白的表达丰度是否在检测范围之内; 2)一抗和二抗是否匹配? 3)一抗和二抗的浓度是否足够? 4)检查抗体是否能够识别样本种鼠中的目标蛋白?
Here you can find Western Blot WB protocol, WB troubleshooting guide, Flow Cytometry FC protocol, Elisa Titre determination, Cell lysate preparation, Immunofluorescence IF Protocol