The troubleshooting tips for high background (uniform distribution) can also be applied to scenarios where non-specific but distinct bands appear on the Western blot membrane. The following should also be cons
Western Blot Troubleshooting In this western blot troubleshooting section, we will help you visually identify specific and common problems on your western blots, such as high background, weak or no signal, multiple bands, uneven staining and sugge...
Many of the challenges that require troubleshooting with traditional Western blots can be overcome with Simple Western™ which automates the traditional steps of Western blots inside of a benchtop instrument. Simple Western uses capillary electrophoresis and eliminates the gel-to-blot transfer of tradit...
I am trying to do a firmware upgrade for my iBlot™ and I am unable to proceed as the Updater seems to be endlessly searching for the iBlot™ device. Can you offer some troubleshooting tips? The iBlot™ devi...
Western Blot Troubleshooting: Why Does The Observed Protein Molecular Weight (MW) Differ From The Calculated One? Western blotting and Molecular Weight (MW) The first WB step is sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), followed...
About western blot antibody non-specific binding problem, multiple bands in membrane as you see, what can one do if there is non-specific binding of primary or secondary antibodies? Reading the following Western Blot troubleshooting guide is supposed to help solving non-specific binding issues in ...
Here Elabscience lists the common Western Blot troubleshooting. 1. No band or low band Possible causes Suggestions The loading amount of sample is low Increase the loading amount The target protein quantity is too low or not expressed in the sample Refer to the relevant literatures to ...
Western Blot Rorschach – Troubleshooting Uneven BackgroundPosted on October 07, 2013 Do you sometimes wonder if you are taking a psychological test when you are trying to interpret your Western blot results? Black spots, smudges and speckles can make it difficult to produce publication quality ...
多用于Western Blot封闭阶段，此外实验条件允许的情况下还可用于Wetern Blot过程中抗体（一抗、二抗）的...
One easy way to increase sensitivity is by reducing background. And the easiest way to do that is to optimize your blocking reagent. “Blocking reagents can have a pretty profound effect on the sensitivity of a blot,” says Short. “Often, this is something that requires optimization and can...