Overcome your Western blot difficulties with our troubleshooting advice, covering problems such as weak/no signal, non-specific bands, high signal, and other common issues.
The troubleshooting tips for high background (uniform distribution) can also be applied to scenarios where non-specific but distinct bands appear on the Western blot membrane. The following should also be considered: Target protein abundance is lower than the threshold of nonspecific binding Load mo...
Western Blot Antibody Dilution Calculator In this western blot troubleshooting section, we will help you visually identify specific and common problems on your western blots, such as high background, weak or no signal, multiple bands, uneven sta...
iBright Imaging Systems What should I do in case of a CCD cooling time issue with the iBright imager? CCD cooling time can be impacted by room temperature and airing/ventilation mechanical blocking. Th...
Western Blot Troubleshooting: Why Does The Observed Protein Molecular Weight (MW) Differ From The Calculated One? Western Blot Troubleshooting: High Background Western Blot Troubleshooting: Weak/No Signal & Other Western Blot ppt Western Blot Video Protocol...
Western Blot Troubleshooting Guide Common problems encountered in western blotting include low signal or high background. This video helps you troubleshoot problems in your Western Blot experiment, and provides solutions to ensure you get the expected results in the shortest amount of time. Read more...
Figure 1: Western blotting after IP – Anti-Light Chain specific antibodies to avoid obscuring analytes in the 50 kDa range. Gels were loaded with Mouse IgG, whole molecule (015-000-003) After SDS-PAGE and transfer to nitrocellulose, blots were blocked with BSA (10% w/v). After incubation...
Western Blot Troubleshooting Common as they are in today’s protein laboratories, Western blots are—let’s face it—a royal pain in the neck. For one thing, the method itself is lengthy and laborious: run the gel, transfer the gel, block, primary antibody, wash, secondary antibody, wash,...
Low signal in western blotting often means poor transfer quality. What are the possible reasons for the poor transfer quality in western blotting and how to prevent weak signal in western blot? The following are Western Blot troubleshooting guide for easy to solve low signal issues, and can help...
The following Western Blot troubleshooting guide is supposed to give you possible causes and corresponding solutions and assist you get reliable results in your western blotting. Western Blot possible causes & solutions for white bands White bands in a Western Blot Possible cause Solution Too much...