Comparison of Western immunoblotting and microimmunofluorescence for diagnosis of Mediterranean spotted fever. One-hundred serum samples from 41 patients suffering from Mediterranean spotted fever (MSF) were tested by microimmunofluorescence (MIF) and Western blot (... N Teysseire,D Raoult - 《Journal...
Comparison of Western blot, counterimmunoelectrophoresis, complement fixation and enzyme-linked immunosorbent assay for the diagnosis of Campylobacter infectiondoi:10.1016/0888-0786(93)90008-NCampylobacterinfectionELISAWesternblottingcomplementfixationtest
Comparison of Dynamic Range Figure 5 compares the dynamic range of the Simple Western to the traditional Western blot. Simon provides a dynamic range over three orders of magnitude for GSK3α in K562 cells, while providing a more linear fit of the data over this range. Figure 5:K562 cells ...
Continue reading: Western Blot Transfer Methods Explore: Transfer Systems Blocking nonspecific sites The membrane supports used in western blotting have a high affinity for proteins. Therefore, after the transfer of the proteins from the ge...
It is worth noting that for the CD63 protein, which is commonly used to detect EVs, western blot results could show a broad band, depending on the post-translational modification of the CD63 protein and the antibody used [31]. In comparison, detection of CD9 and CD81 in EVs using ...
蛋白免疫印迹实验,或Western Blot(又称为免疫印迹,因为使用了抗体来特异性检测其抗原)于1979年由 Towbin 等人提出,现在已是常规的蛋白分析技术。抗体-抗原相互作用的特异性实现了复杂蛋白质混合物中的靶蛋白识别。通过蛋白免疫印迹可获得关于目标蛋白质的定性和...
A comparison of signal emission curves for short- and long duration substrates.When there is an enzyme present in a western blot system, signal output peaks soon after substrate application and rapidly exhausts the substrate (Signa...
A comparison of signal emission curves for short- and long duration substrates.When there is an enzyme present in a western blot system, signal output peaks soon after substrate application and rapidly exhausts the substrate (Signal...
In these example experiments, in which all other conditions were equal, different blocking buffers quenched or enhanced the sensitivity and specificity of the western blot for individual proteins. In other cases, weak blocking ...
In a direct comparison of PVDF vs. nitrocellulose membranes in detecting human immunodeficiency (HIV) in serum, PVDF membrane was shown to hav ter retention of total HIV and improved detection of antibodies to glycosylated envelope (Lauritzen and Pluskal, 1988). See Table 1 for a comparison of...