The line blot, a new immunoassay in which antigens are placed on nitrocellulose as narrow lines, was evaluated for its sensitivity and specificity relative to the microimmunofluorescence assay for the diagnosis of Mediterranean spotted fever (MSF). The line blot assay was only slightly less sensitiv...
Isolation of soluble protein and Western-Blot Analysis Plants materials were grinded at RT by a plastic pestle in an Eppendorf tube of 1.5 mL and resuspended with a BAP buffer containing 50 mM Tris–HCL (pH 7.5), 100 mM ...
(A) Western blot showing a lack of P5CS expression in P5CS-KO vs. WT SH-SY5Y cells (n = 3). β-actin was used as a loading control. (B,C) Effect of METH on glutamate in P5CS-KO cells. (B) The cell extracts were used to measure intracellular glutamate while (C) the ...
The Inno-LIA is a kind of second-generation Western blot and measures antibodies to different HIV antigens in a semi-quantitative way. The pattern and intensity of HIV-specific antibodies both evolve during the first weeks to months after infection. It is thus possible to define algorithms ...
二羟基睾丸脂酮轻微刺激细胞生长,经westernblot检测溶解产物与抗雄性激素受体抗体起免疫反应。EGF刺激细胞生长,但TGFβ-1不能抑制细胞生长。该细胞在裸鼠中成瘤。 NCI-H1395细胞系;细胞传代方法:1:2传代;5-6天传代一次;细胞形态特性:上皮样;多角形;细胞生长特性:贴壁生长;细胞背景资料:详见相关文献介绍 SW579[SW ...
. Using Western blot analysis, we confirmed that RARα deficiency had the predicted differential effect in CL1 and CL2 with a CL1 decrease in CDKN2B protein (p-value = 4.80 × 10−2by paired two-tailed Student’s t-test) and no significant change for CL2 (CL1 vs CL2p <...
Western blot analysis with Odyssey software showing that the Tm in the two types of tumor cells, MDA-MB231 (metastatic) and MCF-7 (nonmetastatic), exhibit different mobilities on SDS–polyacrylamide gels (lower panel). β-Actin was used as a reference for loading (upper panel). Read more ...
EphB4 expression in tumour tissue, surrounding normal tissue, and metastatic lymph node was evaluated by immunohistochemical analysis, western blot, and real-time polymerase chain reaction (PCR). EphB4 expression was then compared between patients based on disease stage and smoking status. The...
Pairwise comparisons of (WT L1 + HELZ2) vs. (M8 [RBM] + HELZ2), p = 0.92ns. Bottom: ORF1p-FLAG and HELZ2 protein levels were detected by western blot using anti-MYC and anti-FLAG antibodies, respectively. GAPDH served as a sample processing control. c HELZ2 ...
All Special IssuesAdvances of Organic Synthesis in Drug DiscoveryAnticancer, Antioxidant, and Anti-Inflammatory Properties of Natural ProductsFeature Annual Reviews in Molecular Sciences 2019Neuroinflammation and Cellular Senescence in Brain Aging and Neurodegeneration: From Molecular Mechanisms to Therapeutic Appl...