Line blot and Western blot immunoassays for diagnosis of Mediterranean spotted fever. J. Clin. Microbiol . 27: 2073–2079.Raoult D, Dasch GA (1989) Line blot and western blot immuno- assays for diagnosis of Mediterranean spotted fever. J Clin Microbiol 27:2073-2079...
血液标本则分别取自大学的相关研究中心和医院;免疫组化(Immunohistochemistry,IHC)技术用于检测组织中 ORF1p 的表达;细胞系实验利用多种细胞系,通过蛋白质免疫印迹(Western blot)等方法研究 ORF1p 的表达和释放;最后,采用单分子阵列(Single Molecule Array,Simoa)检测技术测定患者血液样本中 ORF1p 的含量。
血液标本则分别取自大学的相关研究中心和医院;免疫组化(Immunohistochemistry,IHC)技术用于检测组织中 ORF1p 的表达;细胞系实验利用多种细胞系,通过蛋白质免疫印迹(Western blot)等方法研究 ORF1p 的表达和释放;最后,采用单分子阵列(Single Molecule Array,Simoa)检测技术测定患者血液样本中 ORF1p 的含量。
We evaluated the new blot assay Euroline-WB (ELB, Euroimmun) which consists of a Western blot with Echinococcus multilocularis (E.m.) vesicle antigens and a line blot part with recombinant antigens from E. granulosus (E.g., genus-specific EgAgB) and E.m. (species-specific Em18 and Em...
💥ImageJ 实用技巧分享。拉直 Western Blot 偏斜条带 Western Blot(WB)是目前全球生命科学实验室中使用最广泛的蛋白质分析技术之一。WB 条带常见问题之一是带型异常: 当出现弯曲的条带时应如何使用 Ima - eRebri生物集团-小刘于20240419发布在抖音,已经收获了537个
此细胞系表达前列腺特异抗原。二羟基睾丸脂酮轻微刺激细胞生长,经westernblot检测溶解产物与抗雄性激素受体抗体起免疫反应。EGF刺激细胞生长,但TGFβ-1不能抑制细胞生长。该细胞在裸鼠中成瘤。 3T3-J2细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:详见细胞说明书;细胞生长特性:贴壁;细胞背景资料...
此细胞系表达前列腺特异抗原。二羟基睾丸脂酮轻微刺激细胞生长,经westernblot检测溶解产物与抗雄性激素受体抗体起免疫反应。EGF刺激细胞生长,但TGFβ-1不能抑制细胞生长。该细胞在裸鼠中成瘤。 Nb2-11细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:详见细胞说明书;细胞生长特性:悬浮;细胞背景资料...
Anti-N and anti-M pAbs have also been used for specific detection of SeACoV infection with expected molecular size (42- and 25-KDa, respectively) by western blot (Yang et al., 2019a) or immunohistochemistry (Yang et al., 2019b; Zhou et al., 2018b). In addition, rabbit antiserum ...
EphB4 expression in tumour tissue, surrounding normal tissue, and metastatic lymph node was evaluated by immunohistochemical analysis, western blot, and real-time polymerase chain reaction (PCR). EphB4 expression was then compared between patients based on disease stage and smoking status. The...
Isolation of soluble protein and Western-Blot Analysis Plants materials were grinded at RT by a plastic pestle in an Eppendorf tube of 1.5 mL and resuspended with a BAP buffer containing 50 mM Tris–HCL (pH 7.5), 100 mM NaCl, 250 mM mannitol, 5 mM EDTA, 10% (v/v) glycerol, and pro...