VZV particles are spherical, with a diameter of 180–200 nm. Its genome is approximately 125,000 bp long, including at least 71 open reading frames. The envelope is covered with a variety of glycoproteins, some of which share functions with HSV. The known glycoproteins include gE, gB, gH,...
This virus has been modified by the introduction into its genome of heterologous DNA which encodes an immunogenic polypeptide of another human pathogen. This heterologous polypeptide is expressed in cells infected by the recombinant virus. Such recombinant VZV is useful as a vaccine for chickenpox as...
结果发现4(Fig4):ORF7缺失对病毒复制周期中的侵入和基因组复制表达无显著影响 Fig 4 Role of ORF7 in viral entry, genome replication and expression of typical viralgenes 结果发现5(Fig5-9):pORF7参与了细胞质中的病毒包膜装配。在VZV-7D感染的细胞胞质中分布有大量的包膜缺陷病毒粒子,并且在分化的神经细胞...
but its cell-associated nature has hindered structure studies. Here we report the cryo-electron microscopy structures of purified VZV A-capsid and C-capsid, as well as of the DNA-containing capsid inside the virion. Atomic models derived from these structures show that, despite...
Genome-wide mutagenesis reveals that ORF7 is a novel VZV skin-tropic factor. PMID: 20617166 Varicella-zoster virus: molecular virology and virus-host interactions. PMID: 11495809 Development of recombinant varicella-zoster virus vaccines. PMID: 10599531 ...
ORF 67 and 68 lie adjacent to each other in the unique short region of the VZV genome and code... V Litwin,W Jackson,C Grose - 《Journal of Virology》 被引量: 240发表: 1992年 Demonstration of varicella-zoster virus infection in the muscularis propria and myenteric plexi of the colon ...
FIG. 1. Construction of recombinant VZV with an epitope or stop codons inserted into ORF1. The prototype VZV genome is 124,884 bp in length (line 1) and contains unique long (UL), unique short (Us), terminal repeat (TR), and internal repeat (IR} DNA sequences (line 2). The Notl...
VZV transcriptome profile during lytic and latent infection. Strand-specific mRNA-seq of lytically VZV-infected ARPE-19 cells and seven latently VZV-infected human trigeminal ganglia (TG) (Supplementary Table1).aCircos plots of the VZV genome [purple band; sense and antisense open reading frames ...
The VZV virion contains an icosahedralnucleocapsid surrounding a core that contains the linear, double-stranded DNA genome as well as a protein tegument that separates the capsid from the lipid envelope. This envelope incorporates all of the major viral glycoproteins. The VZV nucleocapsid protein, ...
Therefore, the first 4 specific aims of this proposal are to 1) determine the structure of the SVV genome, 2) identify regions of homology between sVV and DNAs, 3) characterize the sVV glycoproteins, and 4) identify cross-reacting VZV and SVV glycoproteins. This information will provide a ...