The viral genome of VSV has the capacity to accommodate additional genetic material. At least two additional transcription units, totaling 4.5 kb, can be added to the genome, and methods for doing so are known in the art. The added genes are stably maintained in the genome upon repeated ...
Recombinant rVSV-NDV was produced by first modifying a plasmid encoding for the full-length VSV genome (pVSV-XN2) and expressing the F3aa(L289A)-modified fusion protein of NDV (Ebert et al., 2004) as an additional transcription unit between the G and L genes. The endogenous VSV glycopro...
Fig. 1: Generation and characterization of VSV-vectored COVID-19 vaccine candidates. aGenome maps of recombinant VSV vectors. The original VSV contains five transcription units encoding the N, P, M, G, and L genes. The VSV vector was modified by replacing the G gene with a modified SAR...
Stop codons inserted into the p40 subunit of IL-23 in the VSVST viral genome successfully prevented transcription, translation and secretion of IL-23, thus validating the use of this virus as a control. International Journal of Interferon, Cytokine and Mediator Research 2009:1 Powered by TCPDF ...
Image of viral genome and relative sizes of encoded proteins and mRNA. (Source: ViralZone. www.expasy.org/viralzone, Swiss Institute of Bioinformatics)VSV StructureGenome OrganizationPCR sequence of interest from plasmid (Figure 3)Add T7 promoter to PCR templateTranscribe PCR templatePerform Sequence...
(Fig.1A). As these vaccine vectors express two additional proteins encoded in their genome, we expected an attenuation in growth kinetics compared to the parental vector, VSV-EBOV. To test this hypothesis, we analyzed virus replication in triplicate on VeroE6 cells infected with an MOI of ...
Stop codons inserted into the p40 subunit of IL-23 in the VSVST viral genome successfully prevented transcription, translation and secretion of IL-23, thus validating the use of this virus as a control. International Journal of Interferon, Cytokine and Mediator Research 2009:1 Powered by TCPDF ...
Viral entry is the first stage in the virus replication cycle and, for enveloped viruses, is mediated by virally encoded glycoproteins. Viral glycoproteins have different receptor affinities and triggering mechanisms. We employed vesicular stomatitis vir
Transfection of vaccinia-infected mammalian cells with a plasmid encompassing the fusolin sequence plus the upstream regulatory region resulted in transient expression of the gene. This indicated that the vaccinia transcription machinery is able to transcribe the fusolin gene. The fusolin was also ...