The present invention relates to a kind of recombination VSV viruses and viral vectors, its lymphocytic choriomeningitis virus (LCMV) for generating a glycoprotein GP replaces the VSV of G-protein, to virus production cell, and this LCMV-GP-pseudotyped VSV virions and the carrier and cell ...
Adenovirus-mediated recombinant retrovirus generation will be useful in studies with various pseudotyped mutants, as well as in assays for retrovirus-related genes and their products. 展开 关键词: Adenoviridae Gene Expression Regulation, Viral Retroviridae Vesicular stomatitis Indiana virus genetics 腺...
Particularly, to demonstrate the interaction between VSV G and Lgr4, the authors co-expressed the ectodomains of both proteins without their signal peptide (Zhang et al., 2017). Show abstract Reply to Rubinstein: Is Lgr4 essential for VSV- and VSV-G-pseudotyped lentiviral vector entry to ...
(G), and large polymerase subunit (L) to recovery VSV-G pseudotyped ΔG-GFP virus as described in [1]. The antigenomic RNA of ΔG-GFP is expressed from the bacteriophage T7 promoter in pBS, which has been further modified to contain the hepatitis delta ribozyme used to generate a ...
These vectors are typically produced in derivatives of the human 293 cell line and are predominantly pseudotyped with glycoprotein G from vesicular stomatitis virus (VSV-G) (5, 10). In contrast to MLV-based vectors, HIV and FIV VSV-G pseudotyped vectors have demonstrated good transduction ...
Transduction efficiencies of amphotropic and VSV-G-pseudotyped virus for CD34cells did not differ significantly. Gene transfer into CD34CD38cells, which are enriched in more immature progenitors, was not restricted and transfer efficiencies for this subset were also similar for both pseudotypes. The ...
3b) and IC50 values decreased with decreasing amounts of pseudotyped virus input (Fig. 3d, Supplementary Table S1). When using 3.9 × 104 RLU/well of EBOV GP pseudotyped VSV, IC50 of virus neutralisation (0.07 µg/ml) was similar to that expected according to the manufacturer’s ...
The N inth Peopleπs Hospital, School ofM edicine, Shanghai Jiaotong University, Shanghai 200011, China)Abstract: O bjective To obtain high titer ofpseudotyped retroviruses containing GFP gene coated with vesicular stomatitis virus G gly2coprotein(VSV2G) , which could be effectively concentrated ...
Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. PNAS 90: 8033-8037, 1993. Vandepol SB, Lefrancois L, Holland JJ. Sequences of the Major Anitbody Binding Epitopes of the ...
最常用于假型化的包膜蛋白是水泡性口炎病毒(Vesicular Stomatitis Virus)的G蛋白(VSV‑G)。其转导广泛的靶细胞,包括与治疗相关的细胞类型,如干细胞和T 细胞。三聚体VSV‑G蛋白与其受体的结合诱导从融合前状态到融合后状态的多种构象变化, 以催化疏水性融合蛋白插入靶细胞膜以及随后病毒与靶细胞膜的融合。该...