此试剂盒专门用于纯化慢病毒VSV-G pseudotyped Lentivirus。 就像我公司之前推出的用于纯化5型腺病毒试剂盒Vivapure AdenoPACK一样,Vivapure LentiSELECT 40 kit试剂盒基于膜吸附器技术可快速高效纯化细胞悬浮液中的慢病毒。 此试剂盒内的试剂可最佳选择性地吸附和洗脱VSV-G pseudotyped Lentivirus。 Vivapure LentiSELE...
DePolo NJ, Reed JD, Sheridan PL, Townsend K, Sauter SL, Jolly DJ, Dubensky TW Jr: VSV-G pseudotyped lentiviral vector parti- cles produced in human cells are inactivated by human serum. Mol Ther 2000, 2:218-222.DePolo NJ, Reed JD, Sheridan PL, et al. VSV-G pseudotyped lentiviral...
Pseudotyped Lentivirus Vectors Derived from Simian Immunodeficiency Virus SIVagm with Envelope Glycoproteins from Paramyxovirus. JVirol 77: 2607-2614, 2003. 4. Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration...
This protocol is provided for high-titer lentivirus production with Lenti-X Packaging Single Shots (96-well, VSV-G),a kit consisting of 4 x 96-well plates pre-aliquoted with lyophilized Xfect™ Transfection Reagent premixed with an optimized formulation of VSV-G-pseudotyped Lenti-X lentiviral...
[0165] Alexander Otahal,Renate Fuchs,Faisal A.Al‑Allaf,Dieter Blaas(2015) .Release of Vesicular Stomatitis Virus Spike Protein G‑Pseudotyped Lentivirus from the Host Cell Is Impaired upon Low‑Density Lipoprotein Receptor Overexpression.J Virol.2015 Nov;89(22):11723‑6. [0166] Mireille ...
As lentivirus pseudotyped with VSV-G is capable of infecting human cells we strongly recommend BLAST searching the GN18 sequences to both verify that your sequence is unique and to determine if there are potential human targets.另外请问,这个GN18是干什么用的?
Serum from seven animals was analyzed for anti-VSV-G IgG before and after TBI and transplantation with CD34+ HSCs modified with VSV-G-pseudotyped lentivirus. No significant increase in anti-VSV-G IgG was observed following TBI-based conditioning and transplantation (Figure 6A), and no ...
which were then analysed by flow cytometry. No viral binding to mouse cells was seen with any of the pseudotyped vectors (Figure2). It is possible that the scFvs had masked/inactivated the natural receptor-binding site of VSV-G. However, the lack of VSV-G binding is puzzling, as the ...
g假型逆转录病毒载体颗粒或其载体样颗粒(vlp)。 背景技术: 2.在t细胞和干细胞基因疗法的领域,逆转录病毒载体如慢病毒载体(lv)被认为是将治疗性核酸分子递送至靶细胞并诱导长期表达的高效工具。通常,逆转录病毒载体在逆转录病毒膜内含有来自外源病毒物种的异源性包膜蛋白。交换病毒载体包膜蛋白的过程称为“假型化”...
Highly efficient gene transfer into primate-derived embryonic stem (ES) cells has successfully been achieved by using a simian immunodeficiency virus vector (SIV) pseudotyped with V