As a general protocol for transduction, cells were plated at a density of 100,000 cells in a 12-well plate for 24 h and transduced in reduced-serum OptiMEM medium (Gibco) supplemented with 10 µg/mL polybrene (Sigma-Aldrich) at 1:1 (v/v) lentivirus: medium ratio (or 1:100 ...
Fig.1ThevectoroflentivirusfordetectingLC3 EDTA的胰酶消化,种入6孔板中,培养24h。待细胞汇合度达50%左右时,进行换液处理并按照转染试剂LipofectamineTM3000的说明书进行操作。即将VSVG质粒、Δ8.9质粒、RFP-GFP-LC3质粒进行质量浓度1︰1︰1的共转染,转染完成后将细胞置于37℃、5%CO2培养箱中继续培养24h。到达时间...
Enhanced GFP Lentivirus (G418) 数量: 1 供应商: BPS Bioscience Inc. CAS号: / 规格: 500 µl x 2 Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increased ...
Enhanced GFP Lentivirus (Hygromycin) 数量: 1 供应商: BPS Bioscience Inc. CAS号: / 规格: 500 µl x 2 Enhanced green fluorescent protein (eGFP) is a modified (F64L and S65T mutations) version of the native GFP protein isolated from jellyfish (Aequorea victoria), with increa...